In Vitro Characterization of Indigenous Probiotic Strains Isolated from Colombian Creole Pigs

Animals : an Open Access Journal From MDPI
César BetancurBorja Velázquez-Martí

Abstract

Three lactic acid strains were isolated from feces of the native Zungo Pelado breed of pigs (n = 5) and presumably identified as belonging to the Lactobacillaceae family by morphological techniques showing that they were Gram-positive/rod-shaped and catalase- and oxidase-negative. They were then identified by biochemical tests using API 50CHL as Lactobacillus plantarum (CAM6), Lactobacillus brevis (CAM7), and Lactobacillus acidophilus (CL4). However, 16S rRNA identification showed that all three strains were Lactobacillus plantarum. Additionally, all three isolates were able to grow in pH 3 and 4. Interestingly, the growth of the CAM7 strain decreased at pH 5.6 compared to that of the CAM6 strain (p < 0.05), and the growth of the CL4 strain was reduced at pH 7(p < 0.05). All three candidates showed good growth on bile salts (≥0.15%), and CAM6 and CAM7 showed better tolerance at higher concentrations (0.30%). Similarly, all strains tolerated sodium chloride (NaCl) concentrations from 2 to 10%. These strains also grew well at all temperatures tested (30, 37, and 42 °C). The CAM6 strain showed in vitro antibacterial activity against selected enteropathogenic bacteria (Escherichia coli strain NBRC 102203 and Salmonella enterica ser...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Mar 17, 2015·Journal of Animal Science and Biotechnology·Zhaikai ZengXiangshu Piao
Mar 14, 2017·Oxidative Medicine and Cellular Longevity·Paulina Kleniewska, Rafał Pawliczak
Apr 19, 2018·Animal Health Research Reviews·Ximin Zeng, Jun Lin
May 17, 2018·Animal Nutrition·Shengfa F Liao, Martin Nyachoti
Jun 23, 2018·Microbial Genomics·Katelyn Brandt, Rodolphe Barrangou
Nov 15, 2018·BioMed Research International·Md Abul Kalam AzadDan Wan
Jan 9, 2019·Frontiers in Veterinary Science·Christine UnterwegerIsabel Hennig-Pauka
Feb 1, 2019·Scientific Reports·Lesbia Cristina Julio-GonzalezNieves Corzo

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Citations

Jul 28, 2020·Animals : an Open Access Journal From MDPI·César BetancurGuillermo Tellez-Isaias

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Software Mentioned

APIWEB
SPSS
Basic Local Alignment Search Tool

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