PMID: 9429218Jan 16, 1998Paper

In vitro characterization of lectin-induced alterations on the proliferative activity of three human melanoma cell lines

Melanoma Research
P LoréaA Danguy

Abstract

Lectin binding is known to be able to elicit signalling events relevant for various aspects of cell physiology. The influence of lectin binding on melanoma cells remains relatively unexplored. The aim of our study was to investigate the in vitro effects of five plant lectins, namely peanut (PNA), wheat germ (WGA), concanavalin A (Con-A), Griffonia simplicifolia (GSA-IA4) and Phaseolus vulgaris (PHA-L) agglutinins, on the cell proliferation of melanoma cell lines (SK-MEL-28, HT-144 and C32) cultured in media supplemented with either 10% or 1% fetal calf serum (FCS). Cell proliferation was assessed by means of the tetrazolium derivative reduction (MTT) assay. Four lectin concentrations were tested, namely 0.05, 0.5, 5 and 50 micrograms/ml, in four experimental settings, namely 1, 3, 5 and 7 days after the addition of each lectin to the culture media. Determination of the cell gain compartment (percentage of cells in the S and G2 phases of the cell cycle) was done by means of digital cell image analysis assessed on Feulgen-stained nuclei. Our results demonstrated that of the five lectins under study, four had a globally significant dose-dependent toxic effect on melanoma cell proliferation. The fifth lectin, PNA, had a significant...Continue Reading

Citations

Feb 19, 2004·Advanced Drug Delivery Reviews·John D Smart
Sep 24, 1999·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·J D SmartJ D Cook
Sep 27, 2005·Critical Reviews in Food Science and Nutrition·Elvira González De Mejía, Valentin I Prisecaru
Jan 8, 2014·Drug and Chemical Toxicology·Ali JebaliJavad Ranjbari
Apr 28, 2011·Journal of Biochemical and Molecular Toxicology·Glaucia Veríssimo Faheina-MartinsDemetrius Antonio Machado Araujo
Dec 18, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Subhadip MukhopadhyaySujit K Bhutia
Jun 10, 2017·International Journal of Molecular Sciences·Guillaume PoirouxPierre Rougé
Sep 27, 2005·Acta histochemica·Eileen L HeinrichSteven B Oppenheimer
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Oct 13, 2018·Reproduction, Fertility, and Development·Muren HerridJulian A Skidmore
Mar 9, 2019·Frontiers in Oncology·Bradley RyvaReshmi Parameswaran

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