In vitro characterization of naturally occurring influenza H3NA- viruses lacking the NA gene segment: toward a new mechanism of viral resistance?

Virology
V MoulesBruno Lina

Abstract

Among a panel of 788 clinical influenza H3N2 isolates, two isolates were characterized by an oseltamivir-resistant phenotype linked to the absence of any detectable NA activity. Here, we established that the two H3NA- isolates lack any detectable full-length NA segment, and one of these could be rescued by reverse genetics in the absence of any NA segment sequence. We found that the absence of NA segment induced a moderate growth defect of the H3NA- viruses as on cultured cells. The glycoproteins density at the surface of H3NA- virions was unchanged as compared to H3N2 virions. The HA protein as well as residues 188 and 617 of the PB1 protein were shown to be strong determinants of the ability of H3NA- viruses to grow in the absence of the NA segment. The significance of these findings about naturally occurring seven-segment influenza A viruses is discussed.

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Citations

Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Boris EssereVincent Moules
Sep 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Cyrille GavazziRoland Marquet
Oct 10, 2013·Viruses·Edward C Hutchinson, Ervin Fodor
Nov 15, 2011·Nucleic Acids Research·Emilie FournierRoland Marquet
Feb 11, 2012·Antiviral Research·Olivier FerrarisMichèle Ottmann
Nov 17, 2011·Influenza and Other Respiratory Viruses·Gillian M Air
Feb 28, 2017·Scientific Reports·Bernadette Crescenzo-ChaigneSylvie van der Werf
Feb 15, 2019·Frontiers in Immunology·Andrés PizzornoManuel Rosa-Calatrava
Feb 15, 2019·Frontiers in Microbiology·Julie L McAuleyJennifer L McKimm-Breschkin

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