In vitro characterization of the homogalacturonan-binding domain of the wall-associated kinase WAK1 using site-directed mutagenesis

Phytochemistry
Annabelle DecreuxJohan Messiaen

Abstract

Wall-associated kinase 1--WAK1 is a transmembrane protein containing a cytoplasmic Ser/Thr kinase domain and an extracellular domain in contact with the pectin fraction of the plant cell wall in Arabidopsis thaliana (L.) HEYNH. In a previous paper [Decreux, A., Messiaen, J., 2005. Wall-associated kinase WAK1 interacts with cell wall pectins in a calcium-induced conformation. Plant Cell Physiol. 46, 268-278], we showed that a recombinant peptide expressed in yeast corresponding to amino acids 67-254 of the extracellular domain of WAK1 specifically interacts with commercial non-methylesterified homogalacturonic acid, purified homogalacturonans from Arabidopsis and oligogalacturonides in a calcium-induced conformation. In this report, we used a receptor binding domain sequence-based prediction method to identify four putative binding sites in the extracellular domain of WAK1, in which cationic amino acids were selected for substitution by site-directed mutagenesis. Interaction studies between mutated forms of WAK1 and homogalacturonans allowed us to identify and confirm at least five specific amino acids involved in the interaction with homogalacturonan dimers and multimers. The presence of this homogalacturonan-binding domain wit...Continue Reading

Citations

Apr 1, 2008·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Vydehi Kanneganti, Aditya K Gupta
Jan 16, 2009·Carbohydrate Research·Yves VerhertbruggenJ Paul Knox
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Alexandre BrutusGiulia De Lorenzo
Jan 10, 2012·Annual Review of Plant Biology·Sebastian WolfHerman Höfte
Oct 29, 2015·Journal of Experimental Botany·Bruce D Kohorn
Dec 7, 2013·Journal of Integrative Plant Biology·Ying Wu, Jian-Min Zhou
Sep 10, 2009·The Plant Journal : for Cell and Molecular Biology·Bruce D KohornPablo Obregon
Nov 7, 2015·Biochemistry. Biokhimii︠a︡·I A Larskaya, T A Gorshkova
Feb 22, 2014·Molecular Plant·Logan BashlineYing Gu
Dec 9, 2010·Plant Signaling & Behavior·Ivan Spasojević, Jelena Bogdanović Pristov
Sep 19, 2014·Frontiers in Plant Science·Kiwamu TanakaGary Stacey
Aug 19, 2016·Science China. Life Sciences·Lei LiJian-Min Zhou
Oct 27, 2016·BMC Plant Biology·Hyong Woo Choi, Daniel F Klessig
Jan 27, 2016·Molecular & Cellular Proteomics : MCP·Bruce D KohornSusan L Kohorn
Mar 29, 2019·The Plant Journal : for Cell and Molecular Biology·R Glen UhrigWilhelm Gruissem
Mar 1, 2011·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Vydehi Kanneganti, Aditya Kumar Gupta
Dec 13, 2019·Journal of Experimental Botany·Christian Silva-SanzanaFrancisca Blanco-Herrera
Aug 8, 2015·Plant Signaling & Behavior·Bruce D Kohorn
Oct 27, 2017·Molecular Plant-microbe Interactions : MPMI·Yue-Jing GuiXiao-Feng Dai
Feb 6, 2017·The Biochemical Journal·Sebastian Wolf
Oct 18, 2019·Frontiers in Plant Science·Marie-Claire HéloirBenoit Poinssot
Dec 22, 2017·The Plant Journal : for Cell and Molecular Biology·Laura BaceteAntonio Molina
Oct 16, 2018·Frontiers in Plant Science·Jurandir V MagalhaesLeon V Kochian
Aug 15, 2020·The Plant Journal : for Cell and Molecular Biology·Nicholas J LarkanM Hossein Borhan
May 24, 2021·Current Opinion in Biotechnology·Philippe Reymond

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.