In vitro characterization of the mesangial phenotype in a proliferative glomerulonephritis of the rat

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
S HarendzaR A Stahl

Abstract

Mesangial cell proliferation is a predominant feature of many glomerular diseases. We have demonstrated in previous studies that an experimental model of mesangial-proliferative glomerulonephritis in the rat could be transferred to in vitro conditions. Using this model we now have been able to study the mesangial phenotype in several subcultures of mesangial cells from nephritic animals regarding proliferation, and the synthesis of prostaglandins and the matrix degrading enzyme MMP-2. Mesangial-proliferative glomerulonephritis was induced in male Sprague-Dawley rats by a single injection of an anti-Thy 1.1 antiserum. Four days after injection of the antiserum glomeruli were isolated and transferred to tissue culture conditions. Immunohistological characterization of cells, cell growth, synthesis of prostaglandins and expression of MMP-2 were studied in the first and second subculture. Cells from controls and from nephritic animals showed the characteristics of glomerular mesangial cells. Proliferation was decreased in the first and second subculture of cells from nephritic rats compared with controls while there were no immunohistological differences between the cells. Biosynthesis of prostaglandin E2 was significantly increase...Continue Reading

Citations

Mar 7, 2002·The Biochemical Journal·Abdelaziz En-NiaPeter R Mertens
Feb 21, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Naobumi HosoganeTatsuo Suda
Sep 26, 2002·Clinical and Experimental Immunology·N S Sheerin, S H Sacks
Mar 7, 2000·Journal of the American Society of Nephrology : JASN·Oliver LenzWilliam G Stetler-Stevenson
Dec 10, 1999·Journal of the American Society of Nephrology : JASN·P R MertensD H Lovett

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