In vitro combinatory activity of piperine and anti-tuberculosis drugs in Mycobacterium tuberculosis

Tuberculosis
Laíse A HegetoRosilene Fressatti Cardoso

Abstract

Tuberculosis (TB) is an important public health problem worldwide and the emergence of multidrug-resistant (MDR) TB and extensively drug-resistant (XDR) TB worsened the global context. The resistance in Mycobacterium tuberculosis, the causative agent of TB, can partially derive from efflux pumps (EPs) activity in plasma membrane. Due to the recent discovery of piperine (PIP), an organic alkaloid compound, increasing the bioavailability of various drugs, the current assay evaluated the combined activity of PIP and anti-TB drugs in susceptible and resistant M. tuberculosis clinical isolates. The minimum inhibitory concentrations for isoniazid, rifampicin, ethambutol, streptomycin and PIP were determined by resazurin microtiter assay and the combined effects of anti-TB drugs with PIP determined by resazurin drug combination microtiter assay and time-kill curve. The efflux pump inhibitor activity of PIP was determined by bromide accumulation assay and cytotoxicity carried out in VERO cells and J774. A1 macrophages. PIP showed to have EPI activity and RIF + PIP and SM + PIP combinations showed synergistic effect, but low effect in enhancing the killing in M. tuberculosis H37Rv and in the clinical isolates studied, which had differen...Continue Reading

Citations

Sep 5, 2019·Antimicrobial Agents and Chemotherapy·Letícia Sayuri MuraseRosilene Fressatti Cardoso
Jan 25, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Nathally Claudiane de Souza SantosRosilene Fressatti Cardoso
Nov 16, 2018·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Rahul PalZeeshan Fatima
Sep 5, 2020·International Journal of Nanomedicine·Shahab ZomorodbakhshMojgan Sheikhpour
Oct 19, 2019·The Journal of Antibiotics·Melissa MenichiniLaura Rindi
Feb 11, 2021·Immunological Reviews·Gül KilinçMariëlle C Haks
Jun 9, 2021·Future Microbiology·Leonora L CalsavaraKatiany R Caleffi-Ferracioli

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