In vitro communities derived from oral and gut microbial floras inhibit the growth of bacteria of foreign origins.

Microbial Ecology
Xuesong HeWenyuan Shi

Abstract

The gastrointestinal (GI) tract is home to trillions of microbes. Within the same GI tract, substantial differences in the bacterial species that inhabit the oral cavity and intestinal tract have been noted. While the influence of host environments and nutritional availability in shaping different microbial communities is widely accepted, we hypothesize that the existing microbial flora also plays a role in selecting the bacterial species that are being integrated into the community. In this study, we used cultivable microbial communities isolated from different parts of the GI tract of mice (oral cavity and intestines) as a model system to examine this hypothesis. Microbes from these two areas were harvested and cultured using the same nutritional conditions, which led to two distinct microbial communities, each with about 20 different species as revealed by PCR-based denaturing gradient gel electrophoresis analysis. In vitro community competition assays showed that the two microbial floras exhibited antagonistic interactions toward each other. More interestingly, all the original isolates tested and their closely related species displayed striking community preferences: They persisted when introduced into the bacterial commun...Continue Reading

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Citations

Jan 22, 2011·Archaea : an International Microbiological Journal·H-P Horz, G Conrads
Sep 13, 2013·BMC Microbiology·Jing LiMark Stoneking
Mar 23, 2012·International Journal of Food Microbiology·Siele CeuppensMieke Uyttendaele
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Nov 11, 2020·Toxins·Nadja JessbergerErwin Märtlbauer
Nov 8, 2020·Applied and Environmental Microbiology·Yaling JiangDong Mei Deng
Feb 14, 2018·ACS Applied Materials & Interfaces·Yin YangJirun Sun

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Methods Mentioned

BETA
chips
PCR
electrophoresis

Software Mentioned

BLAST
Diversity Fingerprint and Diversity Database Software ( BioRad )
MS Excel

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