In Vitro Community Synergy between Bacterial Soil Isolates Can Be Facilitated by pH Stabilization of the Environment

Applied and Environmental Microbiology
Jakob HerschendMette Burmølle

Abstract

The composition and development of naturally occurring microbial communities are defined by a complex interplay between the community and the surrounding environment and by interactions between community members. Intriguingly, these interactions can in some cases cause synergies, where the community is able to outperform its single-species constituents. However, the underlying mechanisms driving community interactions are often unknown and difficult to identify due to high community complexity. Here, we show how opposite pH drift induced by specific community members leads to pH stabilization of the microenvironment, acting as a positive interspecies interaction, driving in vitro community synergy in a model consortium of four coisolated soil bacteria, Microbacterium oxydans, Xanthomonas retroflexus, Stenotrophomonas rhizophila, and Paenibacillus amylolyticus We use microsensor pH measurements to show how individual species change the local pH microenvironment and how cocultivation leads to a stabilized pH regime over time. Specifically, in vitro acid production from P. amylolyticus and alkali production primarily from X. retroflexus led to an overall pH stabilization of the local environment over time, which in turn resulted i...Continue Reading

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Citations

Oct 23, 2019·Environmental Microbiology·Henriette L RøderMette Burmølle
Feb 1, 2020·PloS One·Poonam NasipuriSøren J Sørensen
Apr 21, 2020·Microbial Biotechnology·Jo De VriezeWilly Verstraete
Sep 12, 2019·Frontiers in Microbiology·Nanna Mee Coops OlsenMette Burmølle
Aug 28, 2020·Frontiers in Cellular and Infection Microbiology·Lasse KvichMads Lichtenberg
Nov 18, 2018·Scientific Reports·Marco KaiBirgit Piechulla

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