In vitro comparison of three different mesh constructions

ANZ Journal of Surgery
Dirk WeyheWaldemar Uhl

Abstract

Material amount and pore size have been recently discussed as probable important determinants of biocompatibility of mesh implants used in hernia repair. This study aimed to find out whether other constructional parameters affect the extent of early foreign body reaction in vitro. An NRK-49F (mixed culture of normal rat kidney cells) fibroblast culture was incubated in the presence of a 'light' microporous mesh (35 g/m(2), 0.25 mm thick), a 'heavy' polypropylene knitted mesh (95 g/m(2), 0.55 mm thick) and a polypropylene/polyglactin composite mesh (35 g/m(2), 0.5 mm thick). A mesh-free cell suspension was used as a control group. Fibroblasts' proliferation, invasion and apoptosis rates were measured by commercially available quantification tests. Levels of tumour necrosis factor-alpha, transforming growth factor-beta1, interleukin (IL)-1 beta, IL-6 and IL-10 secreted by the fibroblasts in the supernatant were dynamically measured in a time kinetics of 6-96 h. Invasion potential as well as proliferation and apoptosis rates of fibroblasts were enhanced by all meshes. The composite mesh stimulated the cell turnover with correspondingly increased levels of IL-6 and suppressed levels of transforming growth factor-beta1 significantly...Continue Reading

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Citations

Sep 4, 2009·International Urogynecology Journal and Pelvic Floor Dysfunction·Tilemachos KavvadiasBernhard Schuessler
Sep 22, 2012·Hernia : the Journal of Hernias and Abdominal Wall Surgery·R A CanutoA Chiaravalloti
Apr 13, 2010·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Peter TakacsCarlos A Medina
Apr 2, 2011·Langenbeck's Archives of Surgery·Karsten JungePetra Lynen Jansen
Jul 23, 2013·Journal of Biomedical Materials Research. Part a·Matthew T WolfStephen F Badylak
Nov 22, 2011·The Journal of Surgical Research·Sean B OrensteinYuri W Novitsky
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Jul 2, 2014·Tissue Engineering. Part C, Methods·Matthew T WolfStephen F Badylak
Jul 9, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Federico VozziClaudio Domenici
Jun 11, 2016·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Alessandro PrudenteCassio Luis Zanettini Riccetto
Sep 25, 2015·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Fernando Goulart Fernandes DiasCassio Riccetto
Nov 24, 2016·Hernia : the Journal of Hernias and Abdominal Wall Surgery·R WiessnerD-U Richter

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