PMID: 9194029May 1, 1997Paper

In vitro confirmation of newly established lung cancer cell lines using flow cytometry and multicellular tumor spheroids

Lung Cancer : Journal of the International Association for the Study of Lung Cancer
S InoueT Ohnuma

Abstract

We report on a simplified method of cytomorphological in vitro confirmation of newly established lung cancer cell lines by using multicellular tumor spheroids (MTS) and flow cytometry (FCM). Eleven cell lines were established from 11 patients with lung cancer. The MTS were produced by culturing cells in agar-coated dish. Cytomorphological studies were made using smears of crushed MTS and frozen sections of MTS. The MTS were fixed doubly with paraformaldehyde and osmic acid for scanning and transmission electron microscopy. Bivariate fluorescence of fluorescein isothyocyanate (FITC, tumor associated antigen, TAA) and propidium iodide (DNA) were measured by FCM. The MTS grew anchorage-independently. Cytopathological and electron microscopic findings of MTS were similar to those of the original clinical specimens. The DNA index and TAA were useful in evaluating the presence or absence of contamination by cells of non-tumor origin. The new cell lines satisfied a minimum of four conditions to confirm their establishment: (a) they originated from humans, (b) they were cytomorphologically identified with specimens from primary lesions, (c) they showed tumorigenicity, and (d) they were free from contamination by cells of different orig...Continue Reading

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Citations

Nov 28, 2002·International Journal of Cancer. Journal International Du Cancer·Alaa-Eldin Salah-EldinMasumi Tsuda
Jul 23, 2003·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Kimihiko OhtsukaAkira Shida
Mar 26, 2002·Cancer Letters·Hiroshi NishiharaKazuo Nagashima
Oct 27, 2001·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·M JinH Kato
Dec 19, 2006·Biochemical and Biophysical Research Communications·Kazuki Omoteyama, Shoichi Inoue
Dec 21, 2000·Japanese Journal of Cancer Research : Gann·A Salah-eldinA Matsuura

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