Abstract
In order to induce esophageal muscle cells' orientation, the silicon wafer with prototype 1 and prototype 2 was designed. Prototype 1 has micro-channels of 200 µm width and 30 µm depth with 30 µm wide wall as the interval. Prototype 2 has channels of 100 µm width and 30 µm depth with a discontinuous wall which has 30 µm gap for each 100 µm channel. The poly(ester urethane) scaffolds with pattern prototype 1 and prototype 2 were fabricated using solution casting method and abbreviated as PU1 and PU2, respectively. Silk fibroin was grafted individually on PU1 and PU2 surface (PU1-SF, PU2-SF) using our previous protocol, aiming at improving scaffolds' biocompatibility. The primary esophageal smooth muscle cell was seeded to evaluate the scaffolds' cytocompatibility in vitro. Characterizations like MTT assay, immunocytochemistry, scanning electron microscope, and Western blotting were applied. After that, poly(ester urethane) scaffolds with double patterns, prototype 1 on the exterior, and prototype 2 in the lumen were implanted into the rabbit esophagous to test the regeneration of the muscle tissue. Results from these preliminary tests showed that the growth and differentiation of primary smooth muscle cells were promoted, but al...Continue Reading
References
May 1, 1994·Circulation·S BanaiE F Unger
Aug 17, 1999·International Journal of Cancer. Journal International Du Cancer·P PisaniJ Ferlay
Dec 12, 2001·Journal of Reconstructive Microsurgery·R MeirerM Siemionow
Oct 31, 2003·Biomaterials·Yabin ZhuJiacong Shen
May 6, 2004·Journal of Biomedical Materials Research. Part a·Yabin ZhuJiacong Shen
Jul 12, 2005·Biomaterials·Yabin ZhuBuddy D Ratner
Jul 19, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Yabin ZhuKerm Sin Chian
Sep 14, 2006·Tissue Engineering·Jin Ye ShenVincent Chan
Nov 4, 2006·Biomaterials·Yabin ZhuKerm Sin Chian
Jan 21, 2009·Biomaterials·Inbar Freeman, Smadar Cohen
Nov 22, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Yuanyuan LiYuxin Liu
Apr 10, 2012·Biotechnology Advances·Purushothaman KuppanUma Maheswari Krishnan
Dec 18, 2012·Journal of Biomaterials Applications·Zhisen ShenYabin Zhu
Jul 3, 2013·ACS Applied Materials & Interfaces·Changfeng GongLin Luo
Aug 7, 2013·European Journal of Cell Biology·Richard ArnoldiChristine Chaponnier
May 27, 2014·Clinical Radiology·S SonavaneS P Singh
Jul 27, 2014·Journal of Biomaterials Applications·Aysel KoçY Murat Elçin
Aug 26, 2014·The Spine Journal : Official Journal of the North American Spine Society·Kenneth R St John
Nov 6, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·Nandana BhardwajNam-Joon Cho
Feb 2, 2015·The Lancet Oncology·Kerm Sin ChianKoji Kono
Jul 26, 2015·Journal of Biomaterials Applications·Lei HouXingyu Liu
Citations
Jul 21, 2016·Experimental Gerontology·John T KimJeffrey C Wolchok
Mar 18, 2017·Expert Opinion on Biological Therapy·Esmond LeeMark W Lowdell
Oct 6, 2020·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·Dimitrios SchizasNikolaos Nikiteas
Jan 25, 2020·Acta Biomaterialia·John KimJeffrey Wolchok