In vitro conversion of sex steroids and expression of sex steroidogenic enzyme genes in amphioxus ovary

Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology
Takanobu MizutaKaoru Kubokawa

Abstract

Sex steroids are essential hormones for reproduction in vertebrates. The existence of a sex steroidogenic pathway in invertebrates is controversial because cytochrome P450 (CYP) genes have not been detected in the genomes of an echinoderm and a urochordate. However, cloning and gene expressions of sex steroid-metabolizing enzymes have been reported in a cephalochordate. In this study, in vitro conversion of sex steroids from pregnenolone (P5) to progesterone (P4), from 17alpha-hydroxyprogesterone (17alpha-P4) to 17alpha, 20beta-dihydroxy-4-pregnen-3-one (17alpha, 20beta-P) and 17alpha, 20alpha-P, and from androstenedione (AD) to estrone (E1), estradiol-17beta (E2), and testosterone (T) were confirmed by an incubation experiment performed using (14)C-labeled precursors and mature ovarian homogenates of the amphioxus Branchiostoma belcheri. In amphioxus, the ability of immature ovaries to synthesize sex steroids was much lower than that of mature ovaries. Post-spawning, the mRNA of CYP11A significantly decreased in females. Transcripts of the CYP19 gene also declined in one-fourth of the females after spawning, although this trend was not supported statistically. The mRNAs of CYP17 and 17beta-HSD did not change before and after s...Continue Reading

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Citations

Aug 23, 2011·Genome Biology and Evolution·Ricard AlbalatMichael Schubert
Nov 21, 2012·PLoS Genetics·Geeta N EickJoseph W Thornton
Apr 26, 2011·The Journal of Steroid Biochemistry and Molecular Biology·G V CallardK A Cotter
Nov 6, 2008·Molecular and Cellular Endocrinology·Ann M TarrantJerzy Adamski
Dec 3, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Michael E BakerRomain A Studer
Jul 27, 2010·Biochemical and Biophysical Research Communications·Michael E Baker

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