PMID: 3310688Sep 1, 1987

In vitro cultivation of third stage larvae of Wuchereria bancrofti to the fourth stage

The American Journal of Tropical Medicine and Hygiene
E D FrankeI Wiady

Abstract

Third stage larvae of Wuchereria bancrofti obtained from laboratory-infected mosquitoes grew and molted to the fourth stage in vitro. The culture medium which supported the best growth and development consisted of a 1:1 mixture (v/v) of two commercially available cell culture media, NCTC 135 and Iscove's modified Dulbecco's medium supplemented with 10% human serum or plasma and an antibacterial/antimycotic mixture. Cultures were incubated at 37 degrees C in an atmosphere of either 5% or 8% CO2 in air. After 35 days of culture, 65% to 100% of the larvae were fourth stage. They were motile and in excellent morphological condition with development of the reproductive system in males and females. This culture system will provide an important tool for biochemical and immunological studies.

References

Jan 1, 1979·The American Journal of Tropical Medicine and Hygiene·J H CrossS Oemijati

Citations

Nov 12, 2017·Parasites & Vectors·Abdel Jelil NjouendouSamuel Wanji
Dec 1, 2009·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·K P PailyP K Das
Jun 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M S Cupp
May 12, 2019·Molecular Biology and Evolution·Scott T SmallPeter A Zimmerman

Related Concepts

Aedes
Tadpole
Genus Wuchereria
Filaria bancrofti

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