In vitro culture conditions favoring selection of chromosomal abnormalities in human ES cells

Journal of Cellular Biochemistry
Marta P ImrehLars Ahrlund-Richter

Abstract

Previous studies in several laboratories have demonstrated inadvertent chromosomal abnormalities in long-term cultured human embryonic stem cells (HESC). Here, using a two-step selection process we report a functional adaptation of a HESC line, HS181, towards a decreased dependence of extra cellular matrix (ECM) for in vitro survival, that is for growth directly onto a plastic surface. Successful adaptation was paralleled with a karyotype change in 100% of the cells to 47,XX,del(7)(q11.2),+i(12)(p10). The resulting adapted population showed increased survival and growth on plastic and also maintained expression of HESC markers, but showed a decreased pluripotency, as demonstrated by results from embryoid body (EB) formation in vitro. The finding of reduced pluripotency may not be totally unexpected since the variant cells were selected for self-renewal and proliferation, not differentiation during the adaptation to growth on plastic. In the light of recent models of a germ cell origin of HESC it is of particular interest that similar to many of the reported spontaneous HESC mutants, one of the identified specific chromosome abnormalities, i(12p), has also been strongly implicated for human germ cell cancer. However, the mutated...Continue Reading

References

Nov 1, 1994·Human Reproduction·A BongsoS Ratnam
Jul 3, 1998·International Journal of Cancer. Journal International Du Cancer·J van EchtenB de Jong
Nov 6, 1998·Science·J A ThomsonJ M Jones
Apr 14, 1999·Scientific American·R A Pedersen
Sep 28, 2001·Nature·R G Edwards
Apr 24, 2003·Reproductive Biomedicine Online·R G Edwards
May 20, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Leendert H J LooijengaJ Wolter Oosterhuis
May 23, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Jirina BartkovaJiri Bartek
Dec 9, 2003·Nature Biotechnology·Jonathan S DraperPeter W Andrews
Jan 6, 2004·Nature Biotechnology·Martin F Pera
Jan 28, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Melissa K CarpenterMahendra S Rao
Jan 28, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Elen S RoslerMelissa K Carpenter
Feb 14, 2004·Human Molecular Genetics·Amander T ClarkRenee A Reijo Pera
Apr 3, 2004·Nature Biotechnology·Jeremy J BuzzardAlan Colman
Jun 10, 2004·Pathology Oncology Research : POR·László Kopper, Melinda Hajdú
Jul 15, 2004·International Journal of Cancer. Journal International Du Cancer·Laurent ChêneAlain Latil
Sep 4, 2004·Stem Cells and Development·Marta P ImrehLars Ahrlund-Richter
Sep 4, 2004·Stem Cells and Development·Karin GertowLars Ahrlund-Richter
Dec 30, 2004·Development·Thomas P Zwaka, James A Thomson
Jan 8, 2005·Nature Biotechnology·Maisam M MitalipovaSteven L Stice
Feb 3, 2005·Cell Research·Chong Feng Gao, George F Vande Woude
May 12, 2005·Lancet·Irina KlimanskayaRobert Lanza
Jun 9, 2005·Nature Biotechnology·Lisa M Hoffman, Melissa K Carpenter
Jul 27, 2005·Seminars in Cancer Biology·Keiran S M SmalleyMeenhard Herlyn

❮ Previous
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Citations

Feb 8, 2008·Bulletin of Experimental Biology and Medicine·M A ProkhorovichN B Rubtsov
Feb 17, 2009·Biogerontology·Jan O Nehlin, Torben Barington
Oct 22, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Hye Won SeolShin Yong Moon
Feb 24, 2010·In Vitro Cellular & Developmental Biology. Animal·Maria A LagarkovaSergei L Kiselev
Jun 6, 2009·Lab on a Chip·Luis Gerardo Villa-DiazGary Daniel Smith
Feb 1, 2011·Stem Cells and Development·Giuseppe R DiaferiaIda Biunno
Dec 12, 2012·Human Reproduction Update·H T NguyenC Spits
Sep 10, 2010·Journal of the Royal Society, Interface·Emma StephensonDusko Ilic
Oct 6, 2012·Clinical Sarcoma Research·Alvin Jm NgCarl R Walkley
Aug 23, 2011·PloS One·Suzanne E PetersonJerold Chun
Nov 12, 2009·Regenerative Medicine·Nathalie LefortMarc Peschanski
Sep 16, 2011·Regenerative Medicine·Andrew L RossJames M Grichnik
Sep 8, 2009·Journal of Visualized Experiments : JoVE·Priscila B CamposStevens K Rehen
Dec 29, 2010·Stem Cell Reviews and Reports·Daniela MoralliEmanuela V Volpi
Jul 24, 2012·Gene·Nestor L Lopez CorralesNadezda Kosyakova
Feb 8, 2011·Differentiation; Research in Biological Diversity·Florence WiannyColette Dehay
Dec 20, 2015·Journal of Negative Results in Biomedicine·Suehelay Acevedo-Acevedo, Wendy C Crone
Jul 19, 2008·Trends in Molecular Medicine·Tamra E Werbowetski-Ogilvie, Mickie Bhatia
Mar 11, 2016·Cellular and Molecular Life Sciences : CMLS·Paola RebuzziniSilvia Garagna
Jul 9, 2009·Journal of Cellular and Molecular Medicine·Abdelkrim HmadchaBernat Soria
Feb 11, 2009·Biotechnology Progress·Danny van NoortHanry Yu
Jun 7, 2006·Biochemical and Biophysical Research Communications·Yanxia LiuHongkui Deng
Jun 9, 2015·Molecular Cytogenetics·Lucie ToscaGérard Tachdjian
Aug 26, 2014·Seminars in Cancer Biology·Isabell HultmanLars Ahrlund-Richter
Aug 9, 2015·Stem Cells Translational Medicine·Natalia VapniarskyKyriacos A Athanasiou

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