In vitro culture increases the frequency of stochastic epigenetic errors at imprinted genes in placental tissues from mouse concepti produced through assisted reproductive technologies

Biology of Reproduction
Eric de WaalMarisa Bartolomei

Abstract

Assisted reproductive technologies (ART) have enabled millions of couples with compromised fertility to conceive children. Nevertheless, there is a growing concern regarding the safety of these procedures due to an increased incidence of imprinting disorders, premature birth, and low birth weight in ART-conceived offspring. An integral aspect of ART is the oxygen concentration used during in vitro development of mammalian embryos, which is typically either atmospheric (~20%) or reduced (5%). Both oxygen tension levels have been widely used, but 5% oxygen improves preimplantation development in several mammalian species, including that of humans. To determine whether a high oxygen tension increases the frequency of epigenetic abnormalities in mouse embryos subjected to ART, we measured DNA methylation and expression of several imprinted genes in both embryonic and placental tissues from concepti generated by in vitro fertilization (IVF) and exposed to 5% or 20% oxygen during culture. We found that placentae from IVF embryos exhibit an increased frequency of abnormal methylation and expression profiles of several imprinted genes, compared to embryonic tissues. Moreover, IVF-derived placentae exhibit a variety of epigenetic profil...Continue Reading

References

Jun 29, 2014·Animal Reproduction Science·Dessie Salilew-WondimKarl Schellander
Aug 1, 2014·Molecular and Cellular Endocrinology·John R McCarrey
Jul 31, 2016·Reproductive Toxicology·Lisa A Vrooman, Marisa Bartolomei
Aug 3, 2016·Genetics, Selection, Evolution : GSE·Pasqualino LoiJohn L Williams
Aug 16, 2016·Fertility and Sterility·Lisa A VroomanMarisa Bartolomei
Aug 30, 2016·Reproductive Biomedicine Online·Rebecca L Kelley, David K Gardner
Sep 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Stella K HurMarisa Bartolomei
Dec 3, 2015·Scientific Reports·Carlee R WhiteMellissa R W Mann
Mar 23, 2018·Stem Cells and Development·Douglas M RudenDaniel A Rappolee
Feb 20, 2018·Molecular Reproduction and Development·Veronique Duranthon, Pascale Chavatte-Palmer
Sep 24, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sebastian CanovasPilar Coy
Dec 7, 2017·Oxidative Medicine and Cellular Longevity·Roberta Ferreira LeiteMarcella Pecora Milazzotto
Dec 1, 2017·PloS One·Roberta ArenaGrażyna Ewa Ptak
Jul 23, 2019·Epigenetics : Official Journal of the DNA Methylation Society·Sneha ManiMonica Mainigi
Dec 5, 2017·European Journal of Human Genetics : EJHG·J C Harperon behalf of the European Society of Human Reproduction and Embryology and European Society of Human Genetics
Jul 3, 2019·Reproductive Biology and Endocrinology : RB&E·Liang ZhaoLifang Sun
Jun 26, 2020·Biology of Reproduction·Chantae Sullivan-PykeMonica Mainigi
Jun 10, 2020·Nature Reviews. Genetics·Zhiyuan Chen, Yi Zhang
May 1, 2017·Journal of Dairy Science·Luiz G B SiqueiraPeter J Hansen
Feb 24, 2019·Scientific Reports·Wael SalemLynda K McGinnis
Jan 1, 2019·Reproduction, Fertility, and Development·Luiz G SiqueiraJoão H Viana
Dec 4, 2017·Human Reproduction Open·J C HarperEuropean Society of Human Reproduction and Embryology and European Society of Human Genetics

Citations

Jan 1, 1992·Molecular Reproduction and Development·Y UmaokaT Mori
Nov 1, 1993·Journal of Reproduction and Fertility·B Fischer, B D Bavister
May 20, 2000·Biology of Reproduction·Adam S DohertyRichard M Schultz
Jan 11, 2002·Developmental Biology·Fátima SantosWendy Dean
May 16, 2003·Biology of Reproduction·Mellissa R W MannMarisa Bartolomei
Oct 30, 2004·Reproductive Biomedicine Online·L KaragencM Bahçeci
Apr 6, 2005·Human Molecular Genetics·Hugh D MorganWolf Reik
Sep 30, 2006·Fertility and Sterility·P F RinaudoRichard M Schultz
Feb 22, 2008·Human Molecular Genetics·Amanda L FortierJacquetta M Trasler
Jun 17, 2008·Fertility and Sterility·Urban WaldenströmStaffan Nilsson
Feb 10, 2009·Fertility and Sterility·Somjate ManipalviratnJames H Segars
Aug 28, 2009·Seminars in Reproductive Medicine·Carter M Owen, James H Segars
Sep 18, 2009·Genes & Development·Marisa Bartolomei
Oct 7, 2009·Human Molecular Genetics·Brenna A Market-VelkerMellissa R W Mann
Feb 2, 2010·Fertility and Sterility·Mai FujiiTakashi Okai
Mar 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jiude MaoCheryl S Rosenfeld
May 24, 2011·Developmental Biology·Folami Y IderaabdullahMarisa Bartolomei
Oct 6, 2011·Molecular and Cellular Endocrinology·John R McCarrey
Mar 24, 2012·Science·Daniel M MesserschmidtBarbara B Knowles
Mar 28, 2012·The Journal of Reproduction and Development·Masashi Takahashi
May 3, 2012·Seminars in Reproductive Medicine·Daniel Skora, David Frankfurter
May 3, 2012·Seminars in Reproductive Medicine·Ali Eroglu, Lawrence C Layman
Jul 18, 2012·Human Molecular Genetics·Eric de WaalJohn R McCarrey
Feb 5, 2013·Fertility and Sterility·Laxmi A Kondapalli, Alfredo Perales-Puchalt
Mar 19, 2013·Cell·Jeannie T Lee, Marisa Bartolomei

Related Concepts

Pathologic Cytolysis
Fertility
Embryo
Human Chorionic Gonadotropin
TMEM43 gene
Hydrogen sulfite
Animal Oviduct
Placenta Previa
DNA Methylation [PE]
Stochastic Processes

Related Feeds

Angelman Syndrome

Angelman syndrome is a neurogenetic imprinting disorder caused by loss of the maternally inherited UBE3A gene and is characterized by generalized epilepsy, limited expressive speech, sleep dysfunction, and movement disorders. Here is the latest research.

Beckwith-Wiedemann syndrome

Beckwith-Wiedemann syndrome is an imprinting disorder characterized by overgrowth, congenital malformations and predisposition to tumors. Discover the latest research on Beckwith-Wiedemann Syndrome here.

Related Papers

Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie
C Bouillon, P Fauque
Reproduction : the Official Journal of the Society for the Study of Fertility
Joshua SommovillaRichard M Schultz
© 2020 Meta ULC. All rights reserved