In vitro culture studies of childhood myelodysplastic syndrome: establishment of the cell line MUTZ-1

Leukemia & Lymphoma
K G SteubeH G Drexler

Abstract

Myelodysplastic syndrome (MDS) in childhood is considered to be very rare and detailed pathobiological data are scarce. More biological information regarding MDS in children is clearly needed and in vitro culture studies provide one possibility for gaining further pathophysiological insights into this malignancy. Here, we incubated bone marrow samples from 30 children with MDS in liquid suspension culture in order to grow the transformed cells in vitro. In most cultures, the hematopoietic cells died quickly and only fibroblastic (stromal) background layers proliferated temporarily; several normal Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cell lines (B-LCL) were established. Only in one instance, albeit from the peripheral blood and not from the bone marrow, could we establish a cell line, termed MUTZ-1, from the malignant cells of a 5-year-old girl with MDS (FAB subtype refractory anemia with excess of blasts). The MDS arose from a pre-existing Fanconi anemia and progressed quickly to an acute myeloid leukemia (FAB M2). Despite positivity for EBV, MUTZ-1 is not an EBV + B-LCL and further characterization of MUTZ-1 confirmed the derivation from the transformed clonal cells. Immunophenotyping showed a pre B-cell surfa...Continue Reading

References

Aug 1, 1992·British Journal of Haematology·H HasleN T Pedersen
Oct 1, 1992·British Journal of Haematology·M A TuncerG J Mufti
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R BanerjeeG Acs
Jul 1, 1992·Archives of Disease in Childhood·I M Hann
Apr 1, 1991·Baillière's Clinical Haematology·J M Chessells
Jan 1, 1991·Acta Paediatrica Hungarica·H Gadner
Jan 1, 1990·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·H D PreislerA A Sandberg
Jan 1, 1990·Annual Review of Immunology·K Dorshkind
Dec 1, 1987·Seminars in Oncology·G J TricotR Hoffman
Jan 1, 1984·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·D HoelzerH Heimpel
Apr 10, 1995·International Journal of Cancer. Journal International Du Cancer·R GiavazziT Barbui
Mar 1, 1995·Leukemia & Lymphoma·J Boultwood, C Fidler
Jun 1, 1995·British Journal of Haematology·Z MarkF Brok-Simoni
May 1, 1994·Leukemia & Lymphoma·D CampanaA Manabe
May 1, 1993·British Journal of Haematology·G H JacksonM M Reid

❮ Previous
Next ❯

Citations

Apr 3, 2010·Hematology/oncology Clinics of North America·Sarah H Beachy, Peter D Aplan
Oct 25, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhaoyun LiuRong Fu
Jul 24, 2008·Journal of the National Cancer Institute. Monographs·Christopher SlapePeter D Aplan
Jul 30, 2002·Leukemia·Z-B HuH G Drexler
Mar 23, 2019·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Lei HuangRong Fu
Sep 29, 2020·Frontiers in Oncology·Qi LvHuaquan Wang
Mar 20, 2021·Animal Models and Experimental Medicine·Weisha LiRan Gao
Apr 7, 2009·Leukemia Research·Hans G DrexlerRoderick A F Macleod

❮ Previous
Next ❯

Related Concepts

Related Feeds

AML: Role of LSD1 by CRISPR (Keystone)

Find the latest rersearrch on the ability of CRISPR-Cas9 mutagenesis to profile the interactions between lysine-specific histone demethylase 1 (LSD1) and chemical inhibitors in the context of acute myeloid leukemia (AML) here.

Acute Myeloid Leukemia

Acute myeloid leukemia (AML) is a clinically and genetically heterogeneous disease with approximately 20,000 cases per year in the United States. AML also accounts for 15-20% of all childhood acute leukemias, while it is responsible for more than half of the leukemic deaths in these patients. Here is the latest research on this disease.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

CNS Lymphoma

In CNS lymphoma, cancerous cells from lymph tissues or other parts of the body form tumors in the brain and/or spinal cord. Here is the latest research on this rare non-Hodgkin lymphoma.

Acute Myeloid Leukaemia & RNA

Acute myeloid leukaemia (AML) is a common hematological type of cancer. As the population ages, there has been a rise in the frequency of AML. RNA expression has been used to see if there are different genetic profiles that exist within AML and whether these may underpin the variations in survival rates. Here is the latest research on AML and RNA.

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.