Abstract
Myelodysplastic syndrome (MDS) in childhood is considered to be very rare and detailed pathobiological data are scarce. More biological information regarding MDS in children is clearly needed and in vitro culture studies provide one possibility for gaining further pathophysiological insights into this malignancy. Here, we incubated bone marrow samples from 30 children with MDS in liquid suspension culture in order to grow the transformed cells in vitro. In most cultures, the hematopoietic cells died quickly and only fibroblastic (stromal) background layers proliferated temporarily; several normal Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cell lines (B-LCL) were established. Only in one instance, albeit from the peripheral blood and not from the bone marrow, could we establish a cell line, termed MUTZ-1, from the malignant cells of a 5-year-old girl with MDS (FAB subtype refractory anemia with excess of blasts). The MDS arose from a pre-existing Fanconi anemia and progressed quickly to an acute myeloid leukemia (FAB M2). Despite positivity for EBV, MUTZ-1 is not an EBV + B-LCL and further characterization of MUTZ-1 confirmed the derivation from the transformed clonal cells. Immunophenotyping showed a pre B-cell surfa...Continue Reading
References
Aug 1, 1992·British Journal of Haematology·H HasleN T Pedersen
Oct 1, 1992·British Journal of Haematology·M A TuncerG J Mufti
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R BanerjeeG Acs
Jul 1, 1992·Archives of Disease in Childhood·I M Hann
Jun 1, 1991·British Journal of Haematology·G BergamaschiE Ascari
Apr 1, 1991·Baillière's Clinical Haematology·J M Chessells
Jan 1, 1991·Acta Paediatrica Hungarica·H Gadner
Jan 1, 1990·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·H D PreislerA A Sandberg
Jan 1, 1990·Annual Review of Immunology·K Dorshkind
Dec 1, 1987·Seminars in Oncology·G J TricotR Hoffman
Jan 1, 1986·Leukemia Research·H G DrexlerJ Minowada
Jan 1, 1984·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·D HoelzerH Heimpel
May 1, 1995·Leukemia & Lymphoma·D H Ryan, J Tang
Feb 1, 1993·Journal of Cellular Physiology·S TohdaM D Minden
Apr 10, 1995·International Journal of Cancer. Journal International Du Cancer·R GiavazziT Barbui
Mar 1, 1995·Leukemia & Lymphoma·J Boultwood, C Fidler
Jun 1, 1995·British Journal of Haematology·Z MarkF Brok-Simoni
Jun 1, 1994·British Journal of Haematology·K TohyamaY Yoshida
Mar 1, 1994·Leukemia & Lymphoma·H Hasle
May 1, 1994·Leukemia & Lymphoma·D CampanaA Manabe
Nov 1, 1993·British Journal of Haematology·T NakagawaK Chihara
May 1, 1993·British Journal of Haematology·G H JacksonM M Reid
Citations
Mar 13, 2014·PloS One·Yingxing YueYao-Hua Song
Apr 3, 2010·Hematology/oncology Clinics of North America·Sarah H Beachy, Peter D Aplan
Nov 22, 2014·PloS One·Weilai XuJie Jin
Sep 1, 2015·PloS One·Jixue ZouKun Zhou
Oct 25, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhaoyun LiuRong Fu
Jul 24, 2008·Journal of the National Cancer Institute. Monographs·Christopher SlapePeter D Aplan
Jul 31, 2008·Acta Pharmacologica Sinica·Jian HuangJie Jin
Jul 30, 2002·Leukemia·Z-B HuH G Drexler
Feb 24, 2001·Leukemia·H XieC A Westbrook
Mar 23, 2019·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Lei HuangRong Fu
Sep 29, 2020·Frontiers in Oncology·Qi LvHuaquan Wang
Apr 1, 2011·International Journal of Hematology·Jian HuangJie Jin
Apr 11, 2015·Stem Cells International·Jixue ZouKun Zhou
Nov 1, 2001·Oncogene·Z HuC A Westbrook
Mar 20, 2021·Animal Models and Experimental Medicine·Weisha LiRan Gao
Feb 2, 2000·Leukemia Research·H G Drexler
Apr 7, 2009·Leukemia Research·Hans G DrexlerRoderick A F Macleod