In Vitro Cytotoxicity and Morphological Assessments of GO-ZnO against the MCF-7 Cells: Determination of Singlet Oxygen by Chemical Trapping

Nanomaterials
Fozia ShaheenSyed Mansoor Ali

Abstract

Graphene-based materials have attracted considerable interest owing to their distinctive characteristics, such as their biocompatibility in terms of both their physical and intrinsic chemical properties. The use of nanomaterials with graphene as a biocompatible agent has increased due to an uptick in dedication from biomedical investigators. Here, GO-ZnO was characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), ultraviolet-visible (UV-Vis) spectroscopy, energy dispersive X-ray analysis (EDAX), and Raman spectroscopy for structural, morphological, and elemental analysis. The toxic extent of GO-ZnO was noted by a methyl-thiazole-tetrazolium (MTT), while cellular morphology was observed towards the MCF-7 cells using an inverted microscope at magnification 40×. The cytotoxic effect of GO-ZnO investigated the cell viability reduction in a dose-dependent manner, as well as prompted the cell demise/destruction in an apoptotic way. Moreover, statistical analysis was performed on the experimental outcomes, with p-values < 0.05 kept as significant to elucidate the results. The generation of reactive oxygen species (ROS) demonstrated the potential applicability of graphene in tumor treatment. These...Continue Reading

References

Jul 21, 2006·Nature·Sasha StankovichRodney S Ruoff
Dec 7, 2010·Toxicology Letters·Yanli ChangHaifang Wang
Apr 14, 2011·Journal of Biomedical Nanotechnology·N V Srikanth VallabaniAlok Dhawan
Jun 10, 2011·ACS Applied Materials & Interfaces·Ken-Hsuan LiaoChristy L Haynes
Jun 18, 2011·Journal of Colloid and Interface Science·G K RameshaS Sampath
Dec 1, 2011·Journal of Visualized Experiments : JoVE·Danli Wu, Patricia Yotnda
Sep 13, 2012·Journal of Nanoscience and Nanotechnology·Se-Mi KangJeong-Woo Choi
Oct 18, 2012·Biomaterials·Sayan Mullick ChowdhuryBalaji Sitharaman
Nov 28, 2012·Advanced Healthcare Materials·Hanene Ali-BoucettaKostas Kostarelos
Jan 30, 2013·Journal of Photochemistry and Photobiology. B, Biology·X C QinB W Yang
Aug 13, 2013·BioMed Research International·Sangiliyandi GurunathanJin-Hoi Kim
Aug 21, 2013·Acta Biomaterialia·Jingquan LiuDusan Losic
Sep 3, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Cenchao ShenBryce N Feltis
Sep 17, 2013·Advanced Materials·Rebeca Romero-AburtoPulickel M Ajayan
Sep 26, 2013·Nanoscale Research Letters·Sangiliyandi GurunathanJin-Hoi Kim
Jan 16, 2014·Chemical Research in Toxicology·Amedea B SeabraNelson Durán
Mar 4, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Nilesh S ChaudhariPrashant K Deshmukh
Aug 6, 2015·Nanomedicine·Fernanda M P TonelliRodrigo R Resende
Sep 1, 2015·Journal of Occupational and Environmental Hygiene·Wei-Chung SuYung Sung Cheng
Oct 16, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Xitong Liu, Kai Loon Chen
Mar 5, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zahra AjdariArbakariya Ariff
Mar 19, 2016·PloS One·Muhammad Hammad AzizZ A Alahmed
Apr 8, 2017·Nanomaterials·Yongchen WangLiang Guo
Jan 21, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chong ChengChangsheng Zhao
Oct 14, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhen HuYudong Huang

❮ Previous
Next ❯

Citations

Apr 10, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Hadi Esmaeili Govarchin GhalehNasrollah Jabbari

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
X-ray
chip

Software Mentioned

Excel

Related Concepts