In vitro cytotoxicity evaluation of a self-adhesive, methacrylate resin-based root canal sealer

Journal of Endodontics
Luigi PinnaFranklin R Tay

Abstract

This study compared the cytotoxicity of MetaSEAL (Parkell Inc, Farmington, NY), a methacrylate resin-based sealer with an epoxy resin-based (AH Plus Jet; Dentsply Caulk, Milford, DE) and a zinc oxide-eugenol-based sealer (Pulp Canal Sealer; SybronEndo, Orange, CA). Five-millimeter diameter disks prepared from the respective sealer and disks prepared from Teflon (negative control) and polymethyl methacrylate (positive control) were placed in direct contact with a rat osteosarcoma (ROS) 17/2.8 rat osteoblast-like cell line at six intervals after setting completely at 72 hours and for 5 succeeding weeks after the disks were immersed in simulated body fluid. Succinate dehydrogenase activity was evaluated by using 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide assay. All sealers exhibited severe toxicity at 72 hours, after which toxicity decreased gradually over the experimental period except for Pulp Canal Sealer, which remained severely toxic. MetaSEAL was more toxic than AH Plus Jet during the first week. Both were similar to the toxicity profile of the positive control after the first week, which was probably diffusion controlled.

References

Oct 1, 1994·Endodontics & Dental Traumatology·J TepelW Hoppe
Jan 1, 1997·Journal of Biomedical Materials Research·Z L SunC T Hanks
Mar 1, 1997·Journal of Endodontics·W R SnyderM C Farach-Carson
Dec 30, 1998·International Endodontic Journal·M Tanomaru FilhoL S Utrilla
Aug 3, 2001·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·W Geurtsen
Apr 19, 2003·International Endodontic Journal·M Bernáth, J Szabó
Sep 27, 2003·International Endodontic Journal·E Schäfer, T Zandbiglari
Sep 25, 2004·Journal of Materials Science. Materials in Medicine·Tsui-Hsien HuangChia-Tze Kao
Jan 24, 2006·Journal of Endodontics·Benjamin A NielsenJ Craig Baumgartner
Apr 28, 2006·International Endodontic Journal·Y-C HoY-C Chang
Aug 22, 2007·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Saziye Sari, Leyla Duruturk
Sep 20, 2007·Journal of Endodontics·Adam DonnellyDavid H Pashley
May 24, 2008·Journal of Endodontics·Gianrico SpagnuoloSandro Rengo
Jun 24, 2008·Journal of Endodontics·Matthew S LawsonRobert J Loushine
Jan 10, 2009·International Endodontic Journal·M G BrackettJ C Wataha

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Citations

Dec 18, 2013·Journal of Endodontics·Elisabetta CottiGabriella Simbula
Mar 15, 2012·Journal of Endodontics·Claudia V BinCarlos Henrique R Camargo
Sep 20, 2011·Journal of Endodontics·Sema BelliGurcan Eskitascioglu
Sep 30, 2010·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Noushin ShokouhinejadMohammad A Sabeti
Mar 12, 2010·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Amer Z AlaneziQiang Zhu
Apr 7, 2009·Journal of Endodontics·Brian R BabbFranklin R Tay
Jul 3, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Martha Goël BrackettJohn C Wataha
Sep 24, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Martha Goël BrackettJohn C Wataha
Jul 20, 2010·International Endodontic Journal·J A CostaM D Sousa-Neto
Feb 4, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Martha Goël BrackettJohn C Wataha
Jul 19, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Martha Goël BrackettJohn C Wataha
Jan 19, 2010·International Endodontic Journal·A S Al-HiyasatH Darmani
Mar 17, 2015·Clinical Oral Investigations·Buket Tuğ KılkışAhmet Serper
Jul 23, 2011·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Keivan ZoufanQiang Zhu
Nov 18, 2015·International Endodontic Journal·F X ReichlF X Reichl
Apr 6, 2013·Journal of Applied Oral Science : Revista FOB·Emmanuel João Nogueira Leal da SilvaAlexandre Augusto Zaia
May 10, 2013·Brazilian Dental Journal·Vagner José Medeiros MartinsRivail Antônio Sérgio Fidel
Dec 5, 2013·Restorative Dentistry & Endodontics·Gautam Pyarelal BadoleShital Gautam Badole
Feb 4, 2010·Brazilian Dental Journal·Mário Tanomaru-FilhoLea Assed Bezerra da Silva
May 20, 2011·Australian Dental Journal·E E Hill, J Lott
Oct 11, 2012·Journal of Oral Science·Esteban G GarzaTakashi Komabayashi
Jan 13, 2018·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Fereshteh ShafieiDordaneh Ghaffaripour
Jul 22, 2014·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Felix Weiser, Michael Behr
Feb 28, 2020·International Journal of Dentistry·Amira KiklySaida Sahtout
Dec 11, 2019·Materials·Diogo Afonso FonsecaManuel Marques Ferreira
Apr 27, 2011·Brazilian Dental Journal·Camila Maggi Maia SilveiraGibson Luiz Pilatti
Apr 30, 2021·Brazilian Dental Journal·Leticia Boldrin MestieriFabiana Soares Grecca
Feb 23, 2010·Journal of Endodontics·Young Kyung KimFranklin R Tay

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