In vitro cytotoxicity of cyclodextrin-bonded birch bark extract

Planta medica
Anke HertrampfRoman Huber

Abstract

Triterpenoids from birch bark, like betulin, seem to have an anticancer potential which needs to be further investigated. Aim of this study was first to explore whether a cyclodextrin-solubilised triterpenoid extract (STE) from birch bark induces selective cytotoxic effects in primary liver cancer cells compared to healthy human hepatocytes. Second, selective cytotoxicity against several tumour cell lines should be analysed. For this purpose, human liver cancer cells derived from mouse xenografts (LIXF 575), healthy human hepatocytes, and 42 different human tumour cell lines were incubated with different concentrations of STE corresponding to 4.3 µM - 137.5 µM betulin (BE). Cytotoxicity was tested with the WST-1 cell proliferation assay, apoptosis with caspase 3/7-activity, and necrosis was determined by the propidiumiodid uptake assay. The pathway of cytotoxic effects was further investigated by immunoblotting of apoptosis inducing factor (AIF) and p53. The monolayer assay was used to analyse selectivity of STE towards different tumour cell lines. STE significantly (p < 0.001) reduced viability and induced apoptosis of LIXF cells in low concentrations corresponding to 8.6 µM BE, while human hepatocytes were affected only in co...Continue Reading

Citations

Dec 4, 2012·International Journal of Molecular Sciences·Codruta SoicaMariana Anghel
Jun 10, 2014·Expert Opinion on Therapeutic Patents·René Csuk
Jul 15, 2015·Natural Product Research·T P ShakhtshneiderV V Boldyrev
Nov 5, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Runlan LuoZeyao Tang
Oct 31, 2020·Natural Product Research·Tomoki IguchiYoshihiro Mimaki

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