PMID: 6983715Nov 1, 1982Paper

In vitro cytotoxicity of mouse macrophages activated by coculture with syngeneic sarcoma cells

Scandinavian Journal of Immunology
R OlstadR Seljelid

Abstract

Normal resident peritoneal macrophages from C3D2 (C3H/Tif X DBA/2) F1 mice were activated in vitro by culturing with semisyngeneic tumour cells. The tumour cells originated from a methylcholanthrene-induced sarcoma (MC1M) growing in vivo in ascites form. Macrophage-mediated cytotoxicity was evaluated after 5 days of in vitro culture, using five different target cells. Semisyngeneic (L 929), allogeneic (B16 melanoma), and xenogeneic (HeLa) tumour cell lines and normal allogeneic fibroblast cell lines (3T3, 3T6) were tested. The morphology and kinetics of the cytotoxicity reaction were studied by scanning electron microscopy and compared with release of radioactivity from 14C-thymidine-labelled target cells. The activated macrophages were able to kill the semisyngeneic, allogeneic, and xenogeneic tumour cell lines tested under conditions that did not affect normal fibroblasts. The requirement for T cells during activation of the macrophages was also tested. The cytotoxicity decreased markedly when T cells were removed from the macrophage cultures before activation or when macrophages from nude mice were used in the experiments.

References

Jan 1, 1979·Advances in Experimental Medicine and Biology·R B HerbermanJ H Dean
Jan 1, 1979·Immunological Reviews·R Kiessling, H Wigzell
Oct 1, 1976·Journal of the National Cancer Institute·M M Stevenson, M S Meltzer
May 29, 1975·Nature·M P FinkW T Shearer
Mar 1, 1971·American Journal of Clinical Pathology·L T YamW H Crosby
Jan 1, 1980·Advances in Pharmacology and Chemotherapy·R M Schultz, M A Chirigos
Sep 1, 1980·The Journal of Experimental Medicine·V H FreedmanS C Silverstein

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Citations

Feb 24, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Samuel BaronKathryn Zoon
May 1, 1985·Pathology, Research and Practice·L Kopper, K Lapis
Oct 1, 1981·Experimental Cell Research·G Kaplan, R Olstad
Jan 1, 1986·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·B PłytyczR Seljelid
May 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L M Reid, D M Jefferson

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