In vitro cytotoxicity of periodontally diseased root surfaces

Journal of Periodontology
L J AdelsonR G Caffesse


The purpose of this experiment was to develop a model system for controlled in vitro testing of root surface toxicity. Human gingival fibroblasts were placed into a depression created in the root surfaces of ten heat sterilized periodontally involved teeth, one half of which were root planed to remove all irritants. The teeth and fibroblasts were incubated in culture medium for 5 days, after which the cells were fixed and stained. Each tooth was examined for migration of cells onto the root planed and non-root planed areas of the tooth, and for evidence of any cytotoxic reaction. No difference in pattern of cell growth, cell migration, or cytotoxic reaction was discernible between root planed and non-root planed areas. Normal fibroblasts could be detected on the surfaces of dentin, calculus, periodontally involved cementum, and enamel. It appears that heat-sterilized periodontally-involved root surfaces, as well as calculus and calculus-laden enamel, were not toxic to human gingival fibroblasts in cell culture.


Mar 1, 1972·Journal of Dental Research·R Snyderman
Dec 1, 1974·Archives of Oral Biology·M LevineD K Mason
Apr 1, 1973·Archives of Oral Biology·M E Neiders, L Weiss
Jan 1, 1969·Current Topics in Microbiology and Immunology·S E MergenhagenH S Shin
May 15, 1970·Science·E HausmannW A Miller
Apr 1, 1971·Archives of Oral Biology·C G Hatfield, A Baumhammers
Jan 1, 1961·Experimental Cell Research·A C TAYLOR
Jan 1, 1965·Journal of Pharmaceutical Sciences·S A ROSENBLUTHJ AUTIAN

❮ Previous
Next ❯


Oct 23, 2002·Journal of Clinical Periodontology·Ahmed Y Gamal, Mohammed M Bayomy
Jun 1, 1988·Journal of Periodontology·L Checchi, G A Pelliccioni
Mar 16, 2004·Journal of Clinical Periodontology·Mahmood KhosraviFazel Shokri
Jun 11, 2003·Journal of Clinical Periodontology·Jörg EberhardSören Jepsen
Sep 1, 1986·Journal of Clinical Periodontology·O FardalE Freeman
Feb 1, 1992·Journal of Periodontology·G Greenstein
Jun 1, 1982·Journal of Periodontology·N M NakibS N Goldstine
Nov 1, 1981·Journal of Periodontology·M R Wirthlin, E B Hancock
Jun 25, 2005·Journal of Periodontology·Amer Sayed-SuleymanThomas Lallier
Aug 28, 2004·Periodontology 2000·Patrick A Adriaens, Laurence M Adriaens
Dec 29, 2000·Journal of Periodontology·C L DriskoUNKNOWN Research, Science and Therapy Committee of the American Accademy of Periodontology
Oct 1, 1995·Periodontology 2000·R A Lowenguth, G Greenstein

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.