In vitro cytotoxicity of silver nanoparticles and zinc oxide nanoparticles to human epithelial colorectal adenocarcinoma (Caco-2) cells

Mutation Research
Yijuan SongXiaoqiang Chen

Abstract

With the increasing applications of silver nanoparticles (Ag NPs) and zinc oxide nanoparticles (ZnO NPs) in foods and cosmetics, the concerns about the potential toxicities to human have been raised. The aims of this study are to observe the cytotoxicity of Ag NPs and ZnO NPs to human epithelial colorectal adenocarcinoma (Caco-2) cells in vitro, and to discover the toxicity mechanism of nanoparticles on Caco-2 cells. Caco-2 cells were exposed to 10, 25, 50, 100, 200μg/mL of Ag NPs and ZnO NPs (90nm). AO/EB double staining was used to characterize the morphology of the treated cells. The cell counting kit-8 (CCK-8) assay was used to detect the proliferation of the cells. Reactive oxygen species (ROS), superoxide dismutase (SOD) and glutathione (GSH) assay were used to explore the oxidative damage of Caco-2 cells. The results showed that Ag NPs and ZnO NPs (0-200μg/mL) had highly significant effect on the Caco-2 cells activity. ZnO NPs exerted higher cytotoxicity than Ag NPs in the same concentration range. ZnO NPs have dose-depended toxicity. The LD50 of ZnO NPs in Caco-2 cells is 0.431mg/L. Significant depletion of SOD level, variation in GSH level and release of ROS in cells treated by ZnO NPs were observed, which suggests tha...Continue Reading

References

Mar 24, 2007·Nanomedicine : Nanotechnology, Biology, and Medicine·Jun Sung KimMyung-Haing Cho
Jan 1, 2008·Small·Nastassja LewinskiRebekah Drezek
Feb 25, 2009·ACS Nano·P V AshaRaniSuresh Valiyaveettil
Jun 10, 2009·Antioxidants & Redox Signaling·Ehab H SarsourPrabhat C Goswami
Aug 25, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Baher Fahmy, Stephania A Cormier
Aug 26, 2009·Annual Review of Public Health·Tian XiaAndre E Nel
Sep 8, 2009·Environmental Science & Technology·Koji KawataSatoshi Okabe
May 4, 2010·Toxicology and Applied Pharmacology·Barbara De BerardisStefania Meschini
Jun 1, 2010·Advanced Materials·Robert LandsiedelWendel Wohlleben
Oct 1, 2005·Nanotechnology·Jose Ruben MoronesMiguel Jose Yacaman
Jun 15, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Maqusood AhamedSalman A Alrokayan
Nov 25, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yi-Yun KaoPei-Shan Liu
Mar 7, 2012·International Journal of Nanomedicine·Mohd Javed AkhtarSalman A Alrokayan
Jun 26, 2012·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·L CalzolaiF Rossi
Sep 22, 2012·Environmental Pollution·Hongbo MaStephen A Diamond
Sep 6, 2013·Mutation Research·Roumen BalanskyAlberto Izzotti
Jul 6, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Rona Miethling-GraffHelmut Erdmann

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Citations

Apr 9, 2016·International Journal of Molecular Sciences·Esther E Fröhlich, Eleonore Fröhlich
Jul 22, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Christie McCrackenW James Waldman
Jul 8, 2015·Toxicology Letters·Sara CastiglioniJeanette A M Maier
Jun 25, 2016·International Journal of Molecular Sciences·Ni ChenHaifang Wang
Aug 10, 2016·Journal of Food Science·Xiaomo MaoAzlin Mustapha
Feb 26, 2016·Scientific Reports·Yuanhao WuQingshui Yin
Nov 6, 2015·International Journal of Molecular Sciences·Myrtill SimkóMats-Olof Mattsson
Mar 2, 2018·Food & Function·Fabiola Moreno-OlivasGretchen J Mahler
Dec 5, 2018·Molecular Nutrition & Food Research·Abigail PodanyShannon L Kelleher
Apr 25, 2019·Journal of Applied Toxicology : JAT·Graziano ColomboFrancesca Caloni
Mar 19, 2020·Critical Reviews in Toxicology·S Keerthana, A Kumar
Oct 9, 2016·Clinical and Experimental Pharmacology & Physiology·Hesham Fathy Hassan HassanBasim Anwar Shehata Messiha
Sep 29, 2017·Frontiers in Microbiology·Chetan SharmaHarsh Panwar
May 18, 2018·Toxicology and Industrial Health·Nisha Bara, Gautam Kaul
Dec 19, 2020·Free Radical Research·Masanori Horie, Yosuke Tabei
Feb 19, 2019·International Journal of Biological Macromolecules·Zhilong YuAzlin Mustapha
Aug 5, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Lei WuYuan Huang
Dec 7, 2018·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Fabiola Moreno-OlivasGretchen J Mahler
Oct 2, 2019·Chemical Research in Toxicology·Monika K LesiówUrszula K Komarnicka

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