In vitro definition of the yeast RNA polymerase I enhancer.

Molecular and Cellular Biology
M C SchultzR H Reeder

Abstract

In vitro conditions are reported under which an EcoRI-HpaI fragment of the Saccharomyces cerevisiae ribosomal gene spacer will enhance transcription from an adjacent RNA polymerase I promoter. Enhancement is largely independent of orientation and distance and is proportional to copy number. Mapping experiments reveal that two separate regions of the EcoRI-HpaI fragment are independently capable of promoter stimulation. These regions appear to correspond to elements which have been shown by previous workers to cause enhancement in vivo. Using the detergent Sarkosyl to limit the number of rounds of transcription from each promoter, we found that the degree of enhancement is similar whether one or many rounds of transcription occur. This finding supports a model in which the enhancer increases the number of stable promoter complexes but does not alter the loading of polymerase on an active promoter. Once the stable promoter complex is formed, the enhancer can be physically severed from the promoter with no loss of enhancement. Likewise, the upstream activation region of the promoter can be severed from the core promoter domain once the stable complex has been formed. These results are interpreted to mean that the enhancer function...Continue Reading

References

Jan 25, 1992·Nucleic Acids Research·S Y ChoeR H Reeder
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M C SchultzR H Reeder
Jan 1, 1990·Progress in Nucleic Acid Research and Molecular Biology·G R Bellomy, M T Record
Nov 25, 1989·Nucleic Acids Research·C A van der SandeR J Planta
Dec 11, 1989·Nucleic Acids Research·W MustersR J Planta
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H Weintraub
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A L GreenleafI R Lehman
Aug 11, 1987·Nucleic Acids Research·L P WoudtR J Planta
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel

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Citations

Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·M C BartonB M Emerson
Mar 17, 2001·Molecular Biology of the Cell·L BordiG Camilloni

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