In vitro dehalogenation of tetrachloroethylene (PCE) by cell-free extracts of Clostridium bifermentans DPH-1

Bioresource Technology
Y C ChangKazuhiro Takamizawa

Abstract

Cell-free extracts of Clostridium bifermentans DPH-1 catalyzed tetrachloroethylene (PCE) dechlorination. PCE degradation was stimulated by addition of a variety of electron donors. Ethanol (0.61 mM) was the most effective electron donor for PCE dechlorination. Maximum activity was recorded at 30 degrees C and pH 7.5. Addition of NADH as a cofactor stimulated enzymatic activity but the activity was not stimulated by addition of metal ions. When the cell-free enzyme extract was incubated in the presence of titanium citrate as a reducing agent, the dehalogenase was rapidly inactivated by propyl iodide (0.5 mM). The activity of propyliodide-reacted enzyme was restored by illumination with a 250 W lamp. The dehalogenase activity was also inhibited by cyanide. The substrate spectrum of activity included trichloroethylene (TCE), cis-1,2-dichloroethylene (cDCE), trans-dichloroethylene, 1,1-dichloroethylene, 1,2-dichloroethane, and 1,1,2-trichloroethane. The highest rate of degradation of the chlorinated aliphatic compounds was achieved with PCE, and PCE was principally degraded via TCE to cDCE. Results indicate that the dehalogenase could play a vital role in the breakdown of PCE as well as a variety of other chlorinated aliphatic comp...Continue Reading

References

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Citations

Jun 17, 2005·Journal of Industrial Microbiology & Biotechnology·Kensuke FurukawaMasatoshi Goto
Mar 26, 2011·Journal of Industrial Microbiology & Biotechnology·Young-Cheol ChangShintaro Kikuchi
Jun 12, 2002·Journal of Bacteriology·Akiko SuyamaKensuke Furukawa
Apr 6, 2017·Environmental Science and Pollution Research International·Iva DolinováAlena Ševců
Jun 18, 2003·Journal of Environmental Quality·Yvette C BerisfordCassandra L Bayer
Jan 7, 2021·Environmental Science and Pollution Research International·Parisa Ebrahimbabaie, John Pichtel

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