PMID: 9538940Apr 16, 1998Paper

In vitro dermal intoxication by bis(chloroethyl)sulfide. Effect on secondary epidermization

Cell Biology and Toxicology
E GentilhommeY Neveux

Abstract

Skin intoxication by bis(beta-chloroethyl)sulfide (BCES; sulfur mustard) induces cutaneous lesions similar to thermal burns, characterized by slowness of skin healing. We have developed an in vitro model of skin equivalent to investigate mechanisms involved in this delay. Direct intoxication of dermal equivalent produced dose- and time-dependent cytotoxicity. A decrease of macroscopic retraction of collagen gels was observed, parallel to the toxic concentration with, at histological level, absence of collagen fiber reorganization. Fibroblast synthesis of fibronectin was also inhibited by intoxication, as demonstrated at an immunobiochemical and immunohistochemical level. These dermal alterations were correlated with secondary modifications of epithelial maturation of nonintoxicated normal human keratinocytes. Cellular adhesion was perturbed, as visualized by a delay in expression and reorganization of basement membrane components, laminen, collagen IV, and fibronectin. Epidermal terminal differentiation was also affected, as shown by the absence of profilaggrin/filaggrin biosynthesis. We demonstrated in vitro, that direct dermal alterations secondarily induce disturbance of epithelial maturation. Taken together, these data show...Continue Reading

Citations

Oct 17, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Michael P ShakarjianJeffrey D Laskin
Mar 12, 2002·The Journal of Investigative Dermatology·D S RosenthalM E Smulson

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