In vitro detection of cells with monocytic potentiality in the wall of the chick embryo aorta

Developmental Biology
F CormierF Dieterlen-Lièvre

Abstract

Our previous investigations in 3- to 4-day avian chimeras have revealed that the wall of the aorta is a site from which hemopoietic stem cells can be obtained. In the present work using an in vitro clonal assay, we searched for cells with monocytic potentiality in this location as well as in the remainder of the embryo's body. In each experimental series thoracic segments from 30 chick embryo aortae were dissociated by a pancreatin treatment and plated in agar medium containing chicken serum and fibroblast-conditioned medium. Eighty to 620 macrophage colonies developed when 50,000 cells from 4-day aortae were plated, somewhat fewer when 3-day cells were plated (19-110). By contrast no progenitors were detected when cells were plated from 3- or 4-day embryos after their aorta had been removed. The cell composition and morphology of colonies deriving from aorta cells, their growth requirement and kinetics of development were identical to these of colonies deriving from young chicken bone marrow cells, cultured in the same conditions. The presence of macrophage progenitors in the wall of the 3- or 4-day embryo aorta and their absence in the rest of the embryo argues for a specific role of that region in embryonic hemopoiesis, name...Continue Reading

References

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Citations

Oct 1, 1995·Anatomy and Embryology·L Pardanaud, F Dieterlen-Lièvre
Jan 1, 1989·Developmental and Comparative Immunology·F Dieterlen-Lievre
Jul 1, 1988·Annales De L'Institut Pasteur. Immunology·P SavagnerJ P Thiery
Jun 1, 1989·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·F YassineF Dieterlen-Lièvre
Apr 12, 2001·British Journal of Haematology·C J Marshall, A J Thrasher
Oct 24, 2002·Development, Growth & Differentiation·Fumie IrahaMitsugu Maéno
May 2, 2002·Journal of Hematotherapy & Stem Cell Research·Adrian J C BloorBerthold Göttgens
Jul 21, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Sarah Al-RoubaieElizabeth A V Jones
Feb 3, 2015·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jason E Fish, Joshua D Wythe
Jun 21, 2014·Experimental Hematology·Aldo Ciau-UitzRoger Patient
Feb 17, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Karine BollerotThierry Jaffredo
Aug 6, 2000·Developmental Biology·T JaffredoF Dieterlen-Lièvre
Nov 21, 2000·Seminars in Immunology·D Dunon, B A Imhof
Sep 11, 2004·Mechanisms of Development·Françoise Dieterlen-Lièvre, Nicole M Le Douarin
Oct 13, 2000·Cell·A Ciau-UitzR Patient
Oct 3, 2006·Developmental Biology·Catherine CorbelFrançoise Dieterlen-Lièvre
Dec 4, 2003·FEMS Immunology and Medical Microbiology·Thierry JaffredoDominique Dunon
Jan 1, 1987·Annals of the New York Academy of Sciences·F Dieterlen-Lièvre
Sep 1, 1990·Experimental Cell Research·F Cormier, F Dieterlen-Lièvre
Apr 5, 2017·Cell and Tissue Research·Yasutaka ImaiMitsugu Maéno
Aug 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K K Anstrom, R P Tucker
May 1, 1994·Circulation Research·M C Fishman, D Y Stainier
Jun 18, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C J MarshallA J Thrasher
Oct 1, 1991·Experimental Cell Research·C Nicolas-BolnetF Dieterlen-Lièvre
Jan 27, 1998·Hematology/oncology Clinics of North America·F Dieterlen-Lièvre

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