PMID: 8600456Mar 1, 1996Paper

In vitro detection of endonuclease IV-specific DNA damage formed by bleomycin in vivo

Nucleic Acids Research
J D Levin, B Demple

Abstract

Endonuclease IV of Escherichia coli has been implicated by genetic studies in the repair of DNA damage caused by the antitumor drug bleomycin, but the lesion(s) recognized by this enzyme in vivo have not been identified. We used the sensitive primer activation assay, which monitors the formation of 3'-OH groups that support in vitro synthesis by E.coli DNA polymerase I, to determine whether endonuclease IV-specific damage could be detected in the chromosomal DNA of cells lacking the enzyme after in vivo treatment with bleomycin. Chromosomal DNA isolated after a 1 h bleomycin treatment from wild-type, endonuclease IV-deficient (nfo-) and endonuclease IV-overproducing (p-nfo; approximately 10-fold) strains all supported modest polymerase activity. However, in vitro treatment with purified endonuclease IV activated subsequent DNA synthesis with samples from the nfo- strain (an average of 2.6-fold), to a lesser extent for samples from wild-type cells (2.1-fold), and still less for the p-nfo samples (1.5-fold). This pattern is consistent with the presence of unrepaired damage that correlates inversely with the in vivo activity of endonuclease IV. Incubation of the DNA from bleomycin-treated nfo- cells with polymerase and dideoxynucl...Continue Reading

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Citations

Apr 15, 2008·Nature Structural & Molecular Biology·Elsa D GarcinJohn A Tainer
Dec 1, 2001·American Journal of Respiratory Cell and Molecular Biology·Y H HeW J Martin
Oct 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christopher M Sassetti, Eric J Rubin
Feb 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Alexander A IshchenkoMurat K Saparbaev
Mar 20, 2010·Journal of Biomedicine & Biotechnology·Ellen S MottaJosé Carlos P De Mattos
Mar 16, 2012·Chemical Biology & Drug Design·Vincent MurrayJon K Chen
Sep 29, 2004·Mutation Research·Jessica L KosaJohn M Essigmann
Jan 1, 1997·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·D Ramotar
Mar 22, 2015·Mutation Research. Reviews in Mutation Research·Miral Dizdaroglu
Dec 17, 2002·Oncogene·Laurent GrosJacques Laval
Dec 17, 2002·Oncogene·Bruce Demple, Michael S DeMott
Dec 12, 2018·Journal of Cellular Physiology·Junfang YanHong Zhang
Mar 24, 2017·Nucleic Acids Research·Elvira ManjónYolanda Sánchez
Jul 12, 2020·Translational Oncology·Vandana SinghFredrik Westerlund
Dec 1, 2011·EcoSal Plus·Susan T Lovett
Jun 14, 2021·DNA Repair·Vandana SinghFredrik Westerlund
Feb 19, 2002·Chemical Reviews·Richard M. Burger

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