PMID: 6094369Jan 1, 1984Paper

In vitro determination of phagocyte activity by luminol- and lucigenin-amplified chemiluminescence

International Journal of Immunopharmacology
R Müller-Peddinghaus

Abstract

Amplified chemiluminescence (CL) detects most sensitively biologically important reactive oxygen species (ROS) which are generated by phagocytes by the respiratory burst permitting the determination of cell activity in vitro. Different murine phagocyte populations were used in combination with various ROS-catabolizing enzymes and some of their inhibitors to determine the possible advantages of one of the two main presently used amplifiers, i.e. luminol and lucigenin. Lucigenin appeared to react mainly with the first of the generated ROS the superoxide anion radical (O-.2) and thus records cell activity via the respiratory burst much more reliable than luminol. The more commonly employed luminol reacts mainly with hydrogen peroxide (H2O2) and probably the singlet oxygen (1O2) which result in photon emission. However, it seems not to react with the hydroxyl radical (OH.). The dependence of luminol-amplified CL upon the generation of the chain reaction intermediate H2O2 and its three main catalysts catalase, myeloperoxidase and glutathione makes this reaction prone to different artifacts if cell activity is to be determined. Lucigenin-amplified CL offers great advantages to study cell activating or inhibiting properties of drugs a...Continue Reading

References

Mar 23, 1978·The New England Journal of Medicine·B M Babior
Jul 1, 1978·Proceedings of the Society for Experimental Biology and Medicine·C P HigginsL A Boxer
Dec 1, 1979·Zeitschrift Für Naturforschung. Section C: Biosciences·E Lengfelder
Jul 1, 1976·Infection and Immunity·R D NelsonP G Quie
Mar 8, 1976·Biochemical and Biophysical Research Communications·R C Allen, L D Loose
Jun 1, 1968·Journal of Bacteriology·S J Klebanoff
Oct 9, 1967·Biochimica Et Biophysica Acta·W H Evans, M Rechcigl
Nov 1, 1981·The Journal of Clinical Investigation·A NakagawaraZ A Cohn
Sep 1, 1983·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·R Mũller-PeddinghausW Schumacher

❮ Previous
Next ❯

Citations

Feb 1, 1991·Biulleten' eksperimental'noĭ biologii i meditsiny·I A ShepetkinV V Udut
Jan 1, 1988·International Journal of Immunopharmacology·P O OgunbiyiP Eyre
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·K A NashA W Thomson
Apr 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D NadeauA Dufresne
Jan 1, 1989·Free Radical Biology & Medicine·V Vilim, J Wilhelm
Jan 1, 1989·Free Radical Biology & Medicine·J A SimpsonR T Dean
Oct 1, 1993·Free Radical Biology & Medicine·K Faulkner, I Fridovich
Nov 9, 2005·International Immunopharmacology·Igor A SchepetkinMark T Quinn
Jul 1, 1992·Scandinavian Journal of Immunology·Z HmamaJ P Revillard
Apr 1, 1992·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·V KaeverK Resch
Nov 1, 1989·Journal of Periodontal Research·A D Gilbert, J A Sofaer
Apr 1, 1989·Journal of Bioluminescence and Chemiluminescence·G Bottu
Jan 1, 1988·Annals of the New York Academy of Sciences·R A FromtlingT P Capizzi
Jun 15, 1991·Biochemical Pharmacology·R RudkowskiG D Champion
Apr 1, 1987·Biochemical Pharmacology·R Müller-Peddinghaus, M Wurl
Jan 1, 1989·Scandinavian Journal of Infectious Diseases·K PauksensP Venge
Jun 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J F WangH de Groot
Apr 11, 2003·Luminescence : the Journal of Biological and Chemical Luminescence·Zenon P CzubaWojciech Krol
Nov 1, 1996·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·C E GerberG Bruchelt
Dec 1, 1985·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·H ErnstG Trautwein
Aug 12, 2009·Analytica Chimica Acta·Marisa FreitasEduarda Fernandes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.