In vitro development of non-enucleated rat oocytes following microinjection of a cumulus nucleus and chemical activation

Zygote : the Biology of Gametes and Early Embryos
Wataru Fujii, Hiroaki Funahashi

Abstract

The present study examined in vitro development and the cytological status of non-enucleated rat oocytes after microinjection of cumulus nuclei and chemical activation. Oocyte-cumulus complexes were collected from gonadotropin-treated prepubertal female Wistar rats 14 h after human chorionic gonadotropin (hCG) injection. Cumulus nuclei were injected into ovulated oocytes and then stimulated in the presence of 5 mM SrCl2 for 20 min at various time points (0-3.5 h) after injection. Some of the reconstituted eggs were cultured to observe the pronuclear formation, cleavage, and blastocyst formation. The incidences of eggs forming at least one pronucleus or containing two pronuclei were not significantly different among the periods (82.4-83.5% and 43.4-51.9%, respectively). Nor did the incidences of eggs cleaving (86.7-97.7%) and developing to the blastocyst stage (0-3.5%) differ depending on when, after injection, stimulation began. When some of the reconstituted eggs were observed for cytological morphology 1-1.5 h after injection, 71.7% of the eggs caused premature chromatin condensation, but only 46.2% of them formed two spindles around each of maternal and somatic chromatins. However, the morphology of the somatic spindles diff...Continue Reading

References

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Citations

Feb 10, 2016·Development, Growth & Differentiation·Islam M SaadeldinByeong Chun Lee
Jan 11, 2008·Biology of Reproduction·Andrea DiLuigiLisa M Mehlmann
Dec 4, 2019·Zygote : the Biology of Gametes and Early Embryos·Xiu-Lan ZhuFeng-Hua Liu

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