In vitro development of placode-derived LHRH neurons: possible involvement of alpha-fetoprotein

Hormones and Behavior
S Daikoku, I Koide

Abstract

Using the olfactory placode (NAP) of 12.5-day-old and vomeronasal organ (VNO) of 14.5-day-old (E12.5, E14.5) rat embryos, we examined in vitro the roles of brain tissues in the development of LHRH neurons. The culture was performed singly or in combination with various brain tissues of E12.5 and E14.5 embryos. Furthermore, a serum-free basal culture medium was supplemented with a water extract of cerebral cortex or septopreoptic-medial basal hypothalamus (S-MBH) of E18.5 embryos and 2-day-old (N2) newborns. In cocultures, many LHRH cells derived from the NAP or VNO migrated into the collocated tissues of the forebrain vesicles and medial basal hypothalamus, especially into the latter, along neural cell adhesion molecule (NCAM)-positive fibers projecting from the NAP and/or the brain tissues, especially in the case of the medial basal hypothalamus. The effects of the brain extracts were evaluated by differentiation, migration, neurite extension, and survival of LHRH neurons. E18.5 S-MBH extract was most effective. In the analysis of the protein composition of the extracts, alpha-fetoprotein (AFP) was determined only in the E18.5 S-MBH extract. When anti-AFP IgG was added to the cultures containing E18.5 S-MBH extract, the stimul...Continue Reading

Citations

Sep 25, 2004·Journal of Neurobiology·Melonie J Storan, Brian Key
Jun 1, 2004·Experimental Biology and Medicine·Gerald J Mizejewski
Dec 12, 2001·Molecular and Cellular Endocrinology·S A TobetG A Schwarting

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