In vitro differentiation and mineralization of dental pulp stem cells on enamel-like fluorapatite surfaces.

Tissue Engineering. Part C, Methods
Xiaodong WangJun Liu

Abstract

Our previous studies have shown good biocompatibility of fluorapatite (FA) crystal surfaces in providing a favorable environment for functional cell-matrix interactions of human dental pulp stem cells (DPSCs) and also in supporting their long-term growth. The aim of the current study was to further investigate whether this enamel-like surface can support the differentiation and mineralization of DPSCs, and, therefore, act as a potential model for studying the enamel/dentin interface and, perhaps, dentine/pulp regeneration in tooth tissue engineering. The human pathway-focused osteogenesis polymerase chain reaction (PCR) array demonstrated that the expression of osteogenesis-related genes of human DPSCs was increased on FA surfaces compared with that on etched stainless steel (SSE). Consistent with the PCR array, FA promoted mineralization compared with the SSE surface with or without the addition of a mineralization promoting supplement (MS). This was confirmed by alkaline phosphatase (ALP) staining, Alizarin red staining, and tetracycline staining for mineral formation. In conclusion, FA crystal surfaces, especially ordered (OR) FA surfaces, which mimicked the physical architecture of enamel, provided a favorable extracellular...Continue Reading

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Citations

Jul 19, 2013·Tissue Engineering. Part a·Xiaodong WangJun Liu
Jun 4, 2016·Biomaterials Research·Sagar N KaushikKyounga Cheon
Mar 11, 2016·Journal of Dental Research·Y LiJ Liu
Aug 21, 2014·Journal of Dental Research·T GuoJ Liu
Jan 23, 2018·Tissue Engineering. Part C, Methods·Laura Macri-PellizzeriVirginie Sottile
Jul 12, 2018·Journal of Dental Research·T GuoJ Liu
Sep 7, 2018·Journal of Dental Research·H L Van der LaanB H Clarkson
Nov 25, 2018·International Endodontic Journal·S HongB Jiang
Oct 19, 2020·Journal of Biomedical Materials Research. Part a·Claudia P MarinEdgar D Zanotto

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Methods Mentioned

BETA
fluorescence imaging
Assay
PCR
fluorescence microcopy

Software Mentioned

Superarray
GraphPad Prism

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