In vitro differentiation of chicken embryo skin cells transformed by Rous sarcoma virus

Journal of Cellular Physiology
M YoshimuraA Kaji

Abstract

The epidermal cells isolated from 14-day chicken embryo shank skin epidermis were infected in vitro with Rous sarcoma virus (RSV). Within a few weeks, rapidly growing colonies of epithelial cells appeared among the sea of transformed fibroblastic cells. When isolated and subcultured, these cells were found to possess typical markers of skin epidermis. The presence of major keratin and typical epithelial cell type morphology strongly suggested that these cells were transformed epidermal cells retaining their differentiated characteristics but having the capacity to propagate in cell culture. If RSV tsNY68, an RSV mutant having a temperature lesion in the src gene, was used, similar transformed epidermal cells were obtained at 36 degrees C (permissive temperature). At the nonpermissive temperature (41 degrees C) the growth rate of these cells decreased and additional keratin species appeared. At 41 degrees C the cells were flattened and lost the refractivity in their peripheries. All the keratins which are synthesized at the nonpermissive temperature were present in normal differentiated shank skin of 19-day old chick embryo. These cells also had "cornified envelop," indicating extensive differentiation. Viral production was as e...Continue Reading

References

Jan 1, 1978·Differentiation; Research in Biological Diversity·S A FelliniH Holtzer
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M MutoA Kaji
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M S Collett, R L Erikson
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·N Kobayashi, A Kaji
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M L Steinberg, V Defendi
Apr 1, 1977·Experimental Cell Research·L B ChenJ K McDougall
Nov 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R O Hynes
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Mar 13, 1980·Biochemical and Biophysical Research Communications·N Kobayashi, A Kaji

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Citations

May 1, 1983·Developmental Biology·M YoshimuraA Kaji
Jan 1, 1989·The International Journal of Biochemistry·E C HeesbeenG E Staal
Dec 1, 1989·Mechanisms of Ageing and Development·A R HeydariA Richardson
Jan 1, 1985·Pharmacology & Therapeutics·D R MakowskiS M Astrin
Feb 1, 1983·The Journal of Cell Biology·S P Banks-Schlegel, P M Howley
Jan 1, 1997·Annual Review of Cell and Developmental Biology·S M Thomas, J S Brugge
Jan 18, 2006·The Journal of Physiology·Georges DaoudJulie Lafond
Jan 31, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Michelle LeonardA Sue Menko

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