PMID: 2504809Jul 1, 1989Paper

In vitro differentiation of fat-storing cells parallels marked increase of collagen synthesis and secretion

Journal of Hepatology
A GeertsE Wisse

Abstract

Fat-storing cells were isolated and purified from livers of normal adult rats and maintained in primary culture. By light and electron microscopy it was established that they underwent phenotypic changes into cells with the ultrastructural characteristics of myofibroblasts, between the third and sixth day in culture. These morphological changes were accompanied by a 2-fold increase of L-[3H]proline incorporation into secretory proteins and an 11-fold increase into secreted collagenase-sensitive proteins. In contrast, incorporation into cell layer-associated proteins and into cell layer-associated collagenase-sensitive proteins was not significantly elevated. Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in combination with fluorography, demonstrated that the main collagen type secreted by the myofibroblast-like cells was collagen type I. Collagen types III and IV, and fibronectin were present in lesser amounts. The similarity between the well known in vivo alterations of fat-storing cells under pathological conditions and the spontaneous in vitro differentiation described in this study, makes primary cultures of fat-storing cells a valuable tool for studying their role in chronic liver disease.

References

Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·G KentH Popper
Mar 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M TsutsumiS Takase
Jul 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S SchäferA M Gressner
Mar 1, 1986·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B ClementA Guillouzo
Apr 1, 1987·Journal of Hepatology·G RamadoriK H Meyer zum Büschenfelde
May 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B ClementA Guillouzo
Sep 1, 1985·Experimental Cell Research·H F HendriksD L Knook
Nov 1, 1985·Proceedings of the Society for Experimental Biology and Medicine·M A LeoC S Lieber
Jul 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Y YokoiK Usui
May 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A M de LeeuwD L Knook
Jan 1, 1983·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M A Leo, C S Lieber
Jul 1, 1983·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Y MinatoJ Takeuchi
Jan 1, 1982·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B VossD B von Bassewitz
Mar 1, 1982·Collagen and Related Research·M Rojkind, P Ponce-Noyola

❮ Previous
Next ❯

Citations

Mar 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J LiC S Lieber
Mar 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A M JonkerJ Grond
Mar 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S L Friedman
Mar 1, 1997·Virchows Archiv : an International Journal of Pathology·M L Hautekeete, A Geerts
Jan 1, 1992·Pharmacology & Therapeutics·M Rojkind
May 7, 2002·Biochemical Pharmacology·Thomas L FreemanMark E Mailliard
Sep 2, 2003·Biochemical Pharmacology·Robert G BennettDean J Tuma
Apr 28, 1999·The Journal of Laboratory and Clinical Medicine·J PoniachikC S Lieber
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·T NakamuraY Okada
Oct 29, 1999·DNA and Cell Biology·R A RippeD A Brenner
Aug 25, 2007·Journal of Gastroenterology and Hepatology·Christopher J ParsonsRichard A Rippe
May 29, 2008·Journal of Gastroenterology and Hepatology·Olav A GressnerAxel M Gressner
Apr 25, 2000·Alcoholism, Clinical and Experimental Research·H OideN Sato
Sep 5, 1992·BMJ : British Medical Journal·A D Burt
Dec 1, 1990·The Journal of Clinical Investigation·B H DavisN O Davidson
Aug 1, 2007·Comparative Hepatology·Olav A GressnerAxel M Gressner
May 1, 1996·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·A Brenzel, A M Gressner
Jan 1, 1993·Cell Motility and the Cytoskeleton·I OgataM Rojkind
Jun 19, 2003·British Journal of Pharmacology·Xuedong ChiChandrashekhar R Gandhi
Dec 4, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Inge MannaertsAlbert Geerts

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
S SchäferA M Gressner
Hepatology : Official Journal of the American Association for the Study of Liver Diseases
A M de LeeuwD L Knook
© 2022 Meta ULC. All rights reserved