In vitro differentiation of human adipose tissue-derived stem cells into cells with pancreatic phenotype by regenerating pancreas extract

Biochemical and Biophysical Research Communications
Jiyeon LeeSong-Cheol Kim

Abstract

Pancreas extract from regenerating pancreas after partial pancreatectomy is known to contain factors that induce islet neogenesis in animals with streptozotocin (STZ)-induced diabetes [A.A. Hardikar, R.R. Bhonde, Modulating experimental diabetes by treatment with cytosolic extract from the regenerating pancreas, Diabetes Res. Clin. Pract. 46 (1999) 203-211]. In this study, we evaluate the effects of regenerating pancreas extract (RPE) from 90% partially pancreatectomized rats on induction of pancreatic differentiation of human adipose tissue-derived stem cells (hASCs). We found that undifferentiated hASCs expressed OCT-3/4, Nanog, and REX-1, markers of embryonic stem cells (ESCs). Genes involved in early pancreas development showed increased expression in RPE-treated culture. Sox17 and IPF-1 were expressed only in RPE-treated culture. Immunocytochemical analysis showed C-peptide-positive cells in RPE-treated culture but not in undifferentiated hASCs. In conclusion, hASCs have the characteristics of ESCs and the potential to differentiate into pancreas cell lineages phenotypically in response to RPE.

References

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Citations

Dec 31, 2010·Surgery Today·Tohru UtsunomiyaHideki Iwaguro
Dec 29, 2012·Journal of Physiology and Biochemistry·P Rahnamay MoshtaghAli M Sharifi
Sep 13, 2012·Journal of Transplantation·Emily Sims, Carmella Evans-Molina
Jun 12, 2010·Biological Chemistry·Jong-Ho ChoiKwang-Hyun Baek
Mar 29, 2014·International Journal of Medical Sciences·Nareshwaran GnanasegaranNoor Hayaty Abu Kasim
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Jul 22, 2014·Journal of Tissue Engineering and Regenerative Medicine·Akram MansouriEsmaeil Ebrahimie
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Aug 1, 2018·Frontiers of Medicine·Shihua WangRobert Chunhua Zhao
Feb 23, 2017·Cellular and Molecular Life Sciences : CMLS·Jane Ru ChoiWan Kamarul Zaman Wan Safwani
Apr 11, 2018·Applied Biochemistry and Biotechnology·Hoveizi Elham, Hashemitabar Mahmoud
Aug 10, 2021·Frontiers in Cell and Developmental Biology·Yanlei YangRobert Chunhua Zhao

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