In vitro DNA binding studies of therapeutic and prophylactic drug citral

International Journal of Biological Macromolecules
Md Fazle AlamHina Younus

Abstract

The study of drug-DNA interactions is of great importance, as it paves the way towards the design of better therapeutic agents. Here, the interaction of DNA with a therapeutic and prophylactic drug citral has been studied. We have attempted to ascertain the mode of binding of citral with calf thymus DNA (Ct-DNA) through various biophysical techniques. Analysis of the UV-visible absorbance spectra and fluorescence spectra indicated the formation of a complex between citral and Ct-DNA. Competitive binding assays with ethidium bromide (EB), acridine orange (AO) and Hoechst 33258 reflected that citral possibly intercalates within the Ct-DNA. These observations were further confirmed by circular dichroism (CD) spectral analysis, viscosity measurements, DNA melting and molecular docking studies. This study is expected to contribute to a better understanding of molecular mechanisms of citral, and design of new drugs in the future.

Citations

Feb 27, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ya-Ping XiaoXiao-Qi Yu
May 14, 2020·Journal of Biomolecular Structure & Dynamics·Shahid Iqbal FarooqiMaham Yamin
Nov 17, 2019·Cell Biochemistry and Biophysics·Amaj Ahmed LaskarHina Younus
Jan 21, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qing-Ying YuXiao-Qi Yu
Oct 8, 2019·Current Cancer Drug Targets·Martyna Godzieba, Slawomir Ciesielski
Apr 29, 2021·Journal of Biomolecular Structure & Dynamics·Dinesh KumarAhmad Ali
Jan 12, 2019·International Journal of Biological Macromolecules·Subharthi BanerjeeKalyan K Mukherjea
Aug 23, 2021·International Journal of Biological Macromolecules·Janmejaya RoutUmakanta Tripathy

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