In vitro dose response to different GPIIb/IIIa-antagonists: inter-laboratory comparison of various platelet function tests

Thrombosis Research
S HarderH K Breddin

Abstract

The aim of this study was to assess the inter- and intra-laboratory variation of the concentration-response to the GPIIb/IIIa-antagonists abciximab and eptifibatide on platelet aggregometry and to compare results with flow cytometric tests as well as the rapid platelet function analyser (RPFA). In five different laboratory sites, blood from three to five healthy donors was spiked with abciximab or eptifibatide, followed by the assessment of: (1) aggregometry (anticoagulant: sodium citrate 3.18% or hirudin 5 microg/ml); (2) flow cytometry (fibrinogen binding or PAC1-expression), or (3) RPFA. Dose-response curves were established on the basis of a sigmoidal Imax)-model [I=(Imax)*Cg)/(IC50g + Cg)]. For citrated blood, aggregation induced by 20 microM ADP was blocked up to 100% by both GPIIb/IIIa-antagonists, IC50 values varied between 0.11-0.22 microg/ml for eptifibatide and 1.25-2.3 microg/ml for abciximab. I(max) of the response to 5 microg/ml collagen ranged from 46% to 100%, and IC50 values varied between 0.28-0.34 microg/ml for eptifibatide and 2.3-3.8 microg/ml for abciximab. In hirudinized blood, IC50 values for eptifibatide were 1.5- to 3-fold higher than those obtained with citrated plasma. Inhibition of PAC1-expression b...Continue Reading

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Citations

Jul 19, 2002·Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions·Nasser LakkisJohn Farmer
Jun 23, 2009·Journal of Thrombosis and Thrombolysis·Nabeel AhmedGraham J Davies
Jan 1, 2008·Future Cardiology·Jochem W van WerkumJurriën M Ten Berg
Aug 23, 2003·Anesthesia and Analgesia·Engelbert DeuschSibylle A Kozek-Langenecker
May 26, 2018·Platelets·Andrew L Frelinger
Feb 5, 2021·Research and Practice in Thrombosis and Haemostasis·Danique L van den KerkhofIngrid Dijkgraaf

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