In vitro effect of chitin particles on the innate cellular immune system of gilthead seabream (Sparus aurata L.)

Fish & Shellfish Immunology
Alberto CuestaJ Meseguer

Abstract

The interaction between chitin particles and gilthead seabream (Sparus aurata L.) head-kidney leucocytes, as well as their effects on the main innate cellular immune responses were studied. Three different chitin particle-sizes were tested: unfiltered, <10 microM and >10 microM. Leucocytes were able to phagocytose only the chitin particles of <10 microM but not the >10 microM ones. Leucocytes were incubated with different concentrations (0 to 1000 microg ml(-1)) of the above chitin particles for 1, 4, 24 or 48 h and their effects on leucocyte viability and the innate cellular immune system were evaluated. Leucocytes incubated with chitin for 48 h maintained their viability as determined by the MTT viability test. Leucocyte phagocytosis of bacteria after chitin incubation for 1 or 4 h was enhanced by the highest chitin concentration tested of each of the chitin fractions studied, while the respiratory burst activity was unaffected. As regards leucocyte natural cytotoxic activity against tumour cells, prior incubation of leucocytes with chitin particles for 1 or 4 h increased while incubation for 24 or 48 h reduced the cytotoxic activity in a dose dependent manner. Statistically significant differences between the different chiti...Continue Reading

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Citations

Oct 18, 2002·Veterinary Immunology and Immunopathology·A CuestaJ Meseguer
Mar 15, 2006·Journal of Fish Diseases·B MagnadottirS Gudmundsdottir
Jun 14, 2005·Fish & Shellfish Immunology·Bergljót Magnadóttir
Feb 12, 2019·Journal of Cellular Physiology·Bharath Kumar VelmuruganChing-Feng Weng
May 29, 2019·Neurology India·Krishna KandarpaKrishnan Ganapathy
Oct 17, 2019·International Journal of Molecular Sciences·Fernando G TorresGiuseppe Bardi
Mar 3, 2017·Clinical Reviews in Allergy & Immunology·Daniel Elieh Ali KomiCharles S Dela Cruz
Oct 29, 2015·Pakistan Journal of Biological Sciences : PJBS·Suriya JongyothaPrawit Suraniranat
Dec 22, 2019·International Journal of Molecular Sciences·Hai-Zhong YuZhan-Jun Lu
Dec 25, 2021·Molecular Ecology·Jamie ParkerOlivia Roth

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