PMID: 6758458Oct 1, 1982

In vitro effect of exogenous insulin on insulin secretion. Studies with glucose, leucine, arginine, aminophylline and tolbutamide

Acta diabetologica latina
E J VerspohlH P Ammon


Using rat pancreatic islets and the perfused rat pancreas, the effect of exogenous insulin on insulin secretion mediated by glucose, leucine, arginine, aminophylline and tolbutamide was studied. (1) In both systems the insulin releasing capacity of glucose was inhibited by exogenous insulin. In the perfused pancreas the inhibition concerned the first and the second phase of insulin release; (2) the EC50 (half-maximal inhibitory effect of insulin on glucose-induced insulin secretion) in islets was 1.2 nM (= 160 microU/ml) and 2.8 nM (390 microU/ml) in perfused pancreas; (3) exogenous insulin also inhibited insulin release in response to leucine and arginine in the isolated islet system and in the perfused pancreas; (4) using aminophylline and tolbutamide in combination with glucose, the extent of the inhibitory effect of insulin was in the range of the inhibitory effect when glucose was used alone as stimulator in islets. Data suggest that the insulinogenic action of physiological stimulators including glucose, leucine and arginine is inhibited by exogenous insulin whereas this seems not to be the case when insulin release was stimulated by aminophylline and tolbutamide. Comparing the EC50s, isolated islets seem to be more sensi...Continue Reading


Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·I D GoldfineA L Jones
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J SchlessingerI Pastan
Nov 1, 1977·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·K ShimaS Tarui
Mar 1, 1978·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·W BeischerE F Pfeiffer
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May 1, 1969·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·L G Heding
May 1, 1970·The American Journal of Physiology·H SandoT Kuzuya
May 1, 1980·The Journal of Clinical Investigation·E J Verspohl, H P Ammon
Feb 1, 1965·Klinische Wochenschrift·H FRERICHSW CREUTZFELDT

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