Abstract
The inhibitory effect of recombinant amaranth cystatin (AhCPI) on the spore germination and growth of the mycotoxigenic fungus Aspergillus parasiticus and Aspergillus niger was investigated. AhCPI showed a concentration-dependent antifungal activity against both fungi. Differential effects were observed when fungi were treated with cystatin in two developmental stages. When AhCPI was added to young mycelium cultures of A. niger, it had a dramatic effect on mycelial growth compared with old mycelium cultures. On the contrary, there was no differential effect of AhCPI addition to either old or young mycelium of A. parasiticus. Furthermore, electron microscopic observations showed that cystatin caused important effects at the level of cell morphology and organelle integrity of both fungi. Additionally, A. parasiticus spores treated with AhCPI presented sensitivity to oxidative, osmotic and ionic stresses; in opposition, under same conditions, A. niger did not show sensitivity to any stressful agent. These results suggest that AhCPI antifungal activity might be related with damage to cell integrity, affecting the survival of the fungi. In addition, our evidences showed that fungal species respond dissimilarly to cystatin; however, ...Continue Reading
References
Jan 1, 1993·Clinical Microbiology Reviews·R F Hector
Jun 4, 1998·Biochemical and Biophysical Research Communications·B N JoshiP K Ranjekar
Feb 17, 2000·FEBS Letters·M PernasG Salcedo
Aug 13, 2002·Applied Microbiology and Biotechnology·E SchusterP W M Van Dijck
Sep 5, 2002·Biochemical and Biophysical Research Communications·A Soares-CostaF Henrique-Silva
Oct 16, 2003·Molecular Plant-microbe Interactions : MPMI·M MartínezI Díaz
Jul 17, 2004·Microbiology·David J Adams
Sep 8, 2006·International Journal of Antimicrobial Agents·Stephanie St George, Claude P Selitrennikoff
Nov 14, 2006·Journal of Experimental Botany·Zamira AbrahamIsabel Diaz
Sep 18, 2007·Plant Physiology and Biochemistry : PPB·Silvia Valdés-RodríguezJohn Délano-Frier
Sep 13, 2008·The FEBS Journal·Ke-Ming WangKai-Wun Yeh
Apr 21, 2010·Plant Physiology and Biochemistry : PPB·Silvia Valdes-RodriguezArmando Guerrero-Rangel
Jun 19, 2010·Biochimie·Meriem BenchabaneDominique Michaud
Nov 18, 2010·Plant Cell Reports·Laura CarrilloIsabel Díaz
Aug 2, 2011·Microbial Cell Factories·Thomas WucherpfennigRainer Krull
Aug 28, 2012·Fungal Genetics and Biology : FG & B·José L Cabrera-PonceJosé Ruiz-Herrera
Feb 20, 2013·Advances in Genetics·Stephen J Free
Dec 1, 2014·Botanical Studies·Mei-Ling ChengWing-Ming Chou