PMID: 589457Dec 2, 1977

In vitro effects of chlorpromazine on microtubules and the Golgi complex in embryonic chick spinal ganglion cells: an electron microscopic study

Brain Research
J ThybergA Hinek

Abstract

Spinal ganglia from 11-day-old chick embryos were incubated in media containing chlorpromazine (CPZ), lidocaine or D-amphetamine and subsequently examined by transmission electron microscopy. CPZ and lidocaine both caused a structural modification of the neuroblasts similar to that induced by the microtubular-disrupting drugs colchicine and vinblastine. That is, there was a partial disappearance of cytoplasmic microtubules with a concomitant increase in the number of microfilaments and the dictyosomes of the Golgi complex changed, with the number of narrow cisternae decreasing and the number of associated vacuoles increasing. Moreover, the dictyosomes were more distinctly separated from each other than in control ganglia. D-Amphetamine did not give rise to any clear changes in either the microtubular system or the Golgi complex and, furthermore, opposed the colchicine-like effects of CPZ. On the basis of these results it is suggested that the subcellular mechanism of action of CPZ involves the cytoplasmic microtubular system and thus secondarily leads to structural and functional alterations in the Golgi complex, viz., in the case of nerve cells, a disturbance in the production and packaging of material destined for the axon te...Continue Reading

References

Feb 1, 1976·Journal of Ultrastructure Research·S MoskalewskiU Friberg
Jan 1, 1976·Annual Review of Biochemistry·J A Snyder, J R McIntosh
Jan 1, 1976·Annual Review of Pharmacology and Toxicology·F E Samson
Mar 24, 1972·Biochemical and Biophysical Research Communications·J A FerrendelliD M Kipnis
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Jul 25, 1958·The Journal of Biophysical and Biochemical Cytology·M L WATSON

Citations

Jul 1, 1985·Experimental Cell Research·J Thyberg, S Moskalewski
Apr 29, 2015·Clinical Psychopharmacology and Neuroscience : the Official Scientific Journal of the Korean College of Neuropsychopharmacology·Ganesan Venkatasubramanian

Related Concepts

Chick Embryo
Thorazine
Oxydess
Dose-Response Relationship, Drug
Ganglia, Spinal
Golgi Apparatus
Lidocaine Monohydrochloride, Monohydrate
Microtubules

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