PMID: 9442827Jan 27, 1998Paper

In vitro effects of diazepam on human ciliary function

Acta Oto-laryngologica
M JohnstonA Drake-Lee

Abstract

This study demonstrated the in vitro effect of diazepam on human ciliary beat frequency (CBF) from fifteen normal subjects using brush cytology. Tube A contained culture medium, tube B the diluent that diazepam intravenous injectate was carried in and culture medium (controls). Tube C, D and E contained, 0.4 mg/l, 4.0 mg/l and 40.0 mg/l of diazepam in culture medium respectively. The mean effective diazepam concentration in plasma is 0.4 mg/l. CBF was measured photometrically. The most vigorous cilia were measured in 5 areas taking 10 readings on each sample, 30 minutes and 1 hour after mixing. Standard deviation (SD) and confidence limits were calculated along with significance testing (p < 0.05) using the paired t-test and ANOVA. The mean of the CBF of tubes A and B were 13.44 (SD 2.65) and 13.67 (SD 2.48). There was a reduction of the CBF with increasing concentrations of diazepam at 30 minutes, 11.32 (SD 2.14), 10.29 (SD 1.58) and 4.14 (SD 1.57) tubes C. D and E respectively. There was a significant lowering in CBF of 17% (p < 0.01) of diazepam at the mean effective plasma level (tube C) when compared against the controls. CBF decreased over time and at 1 hour was 10.57 (SD, 1.36), 9.02 (SD, 1.39) and 3.58 (SD, 1.31) tubes ...Continue Reading

References

Apr 1, 1992·Clinical Otolaryngology and Allied Sciences·A M RobsonA B Drake-Lee
Apr 1, 1992·Thorax·A HasaniS W Clarke
Aug 1, 1991·The Journal of Physiology·G Di BenedettoA Mehta
Jan 1, 1990·Annual Review of Physiology·P Satir, M A Sleigh
Jan 1, 1990·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·K J IngelsE H Huizing
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E H CantorS Spector
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·W C Taft, R J DeLorenzo
Jan 1, 1981·Cell Motility·E R Dirksen, M Zeira
Apr 1, 1995·Clinical Otolaryngology and Allied Sciences·A GreenA B Drake-Lee
Feb 1, 1994·Archives of Otolaryngology--head & Neck Surgery·K J IngelsE H Huizing
Jul 1, 1993·British Journal of Anaesthesia·C S Goodchild

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Citations

Mar 11, 2006·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Hiroko IidaKazuhiko Fukuda
Dec 17, 2014·American Journal of Rhinology & Allergy·Alan D Workman, Noam A Cohen
Jun 28, 2007·Brain, Behavior and Evolution·Catalina SueiroIsabel Rodríguez-Moldes

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