In vitro effects of high glucose concentrations on membrane protein oxidation, G-actin and deformability of human erythrocytes

Cell Biochemistry and Function
Halil ResmiGül Güner

Abstract

The object of this study was to examine the effect of elevated in vitro glucose concentrations on protein modification and functional changes in human erythrocytes. Groups were exposed to 5-45 mM glucose concentrations. The time effect of any changes was also evaluated. In erythrocyte ghosts, protein glycation and oxidation were evaluated using spectrophotometric methods. G-actin was measured by a DNase I inhibition assay in cell lysates. Erythrocyte deformability was assessed using a cell transit analyser. At 24 h, a significant protein oxidation (at 25 and 45 mM glucose; p < 0.05), and G-actin increase was observed for all concentrations (p < 0.05). At 48 h, a significant increase in glycation (25 and 45 mM glucose; p < 0.05), protein oxidation (p < 0.05), and G-actin (p < 0.05) was observed in all groups. A significant positive correlation was observed between glucose /protein oxidation, glucose/G-actin and protein oxidation/G-actin at 24 and 48 h. Our findings show that the oxidative effect of glucose on erythrocytes depends on concentration and incubation time. We also present the first evidence of increased G-actin in human erythrocytes exposed to high glucose concentrations as a diabetes model.

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Citations

Feb 14, 2013·The Review of Diabetic Studies : RDS·Leonid LivshitsRoy Eldor
Jul 5, 2015·Redox Biology·Jana ViskupicovaIzabela Sadowska-Bartosz
Dec 1, 2012·Current Opinion in Cell Biology·Jonathan R Terman, Anna Kashina
May 29, 2015·International Urology and Nephrology·M PappaK Katopodis
Mar 18, 2008·Molecular & Cellular Proteomics : MCP·Erica M PasiniAlan W Thomas
Nov 29, 2017·International Journal of Molecular Sciences·Alena SobolevaAndrej Frolov
Jul 3, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Oliver Baum, Marius Bigler
Dec 22, 2006·Nature Clinical Practice. Nephrology·Merlin C Thomas
Apr 6, 2021·Frontiers in Cell and Developmental Biology·Shamulailatpam Shreedarshanee DeviRanjana Arya
Jun 3, 2021·International Journal of Molecular Sciences·Chloé TurpinPhilippe Rondeau

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