In vitro effects of mammalian leptin, neuropeptide-Y, beta-endorphin and galanin on transcript levels of thyrotropin beta and common alpha subunit mRNAs in the pituitary of bighead carp (aristichthys nobilis)

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Indrajit ChowdhuryJohn Yuh-Lin Yu

Abstract

Thyrotropin (thyroid stimulating hormone, TSH) is a member of the pituitary glycoprotein hormones, consisting of two dissimilar subunits, alpha and beta. The two subunits are produced by different genes and are regulated independently. We have previously cloned a TSHbeta cDNA from bighead carp pituitary and investigated its gene regulation. We report here the direct effects of mammalian TSH-releasing hormone (TRH), leptin, neuropeptide-Y (NPY), beta-endorphin and galanin on mRNA levels of both TSHbeta and alpha-subunits in the pituitary of bighead carp in vitro. The dispersed pituitary cells of bighead carp were incubated at 25 degrees C for 6 h with different doses of these factors. The relative mRNA levels of TSHbeta and alpha-subunits were estimated by traditional polymerase chain reaction (PCR) analysis and fluorescence real-time PCR analysis. The results revealed that mammalian TRH, leptin and beta-endorphin produced dose-dependent stimulatory effects on mRNA levels of both TSHbeta and alpha-subunits while thyroxine (T4) and mammalian galanin suppressed mRNA levels of both TSHbeta and alpha-subunits. NPY suppressed TSHbeta mRNA level, but stimulated alpha-subunit mRNA level. This study has demonstrated that mammalian TRH, ...Continue Reading

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Citations

Oct 1, 2013·General and Comparative Endocrinology·Shan HeYa-Xiong Tao
Oct 8, 2013·General and Comparative Endocrinology·R A JonesD S Mackenzie
Jan 13, 2009·General and Comparative Endocrinology·Duncan S MacKenzieThomas C Miller
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Aug 29, 2012·General and Comparative Endocrinology·Shuge WuWensheng Li
Jan 15, 2008·General and Comparative Endocrinology·M YacobovitzA Gertler
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Apr 15, 2017·Environmental Science and Pollution Research International·Martin StaroňPeter Javorský
Dec 13, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·John Yuh-Lin YuYung-Hsi Kao
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Jul 2, 2019·Frontiers in Endocrinology·Marco António Campinho
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Oct 13, 2020·General and Comparative Endocrinology·Karine RousseauSylvie Dufour
Jun 24, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Roger IzigaManuel Manchado

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