PMID: 2492475Feb 1, 1989Paper

In vitro effects of sulfonylurea on glucose transport and translocation of glucose transporters in adipocytes from streptozocin-induced diabetic rats

Diabetes
D B JacobsD H Lockwood

Abstract

The in vitro effects of the sulfonylurea glyburide on insulin binding and action were compared in adipocytes from control and nonketotic streptozocin-induced diabetic rats. Adipose tissue from control and diabetic animals was maintained in the absence or presence of 2 micrograms/ml glyburide for 20 h. Insulin binding and insulin-stimulated glucose transport were examined in adipocytes prepared from this tissue. As expected, insulin binding was increased in adipocytes from diabetic animals. Exposure of tissue to glyburide did not influence insulin binding in either control or diabetic cells. Glucose transport activity of diabetic cells, assessed with 2-deoxyglucose, was decreased 30-40% in both the absence (basal) and presence of insulin compared with controls. Glyburide potentiated insulin's effects in both control (15-20%) and diabetic (30-40%) adipocytes. As a result, glucose transport activity in glyburide-treated diabetic cells was restored to a level similar to that of control cells not exposed to the drug. The mechanism by which glyburide potentiated glucose transport activity was examined with the D-glucose-displaceable cytochalasin B-binding technique to measure glucose-transporter concentration in membranes prepared fr...Continue Reading

Citations

Oct 1, 1990·Acta diabetologica latina·R GomisW J Malaisse
Mar 2, 2002·Journal of Diabetes and Its Complications·Christoph Rosak
Dec 31, 2002·Adverse Drug Reactions and Toxicological Reviews·Maribel Salas, Jaime J Caro
Apr 7, 2007·Biopharmaceutics & Drug Disposition·V StetinováM Prazáková
May 14, 2009·Journal of Veterinary Medical Education·Catriona E BellSusan M Rhind
Oct 1, 1993·Diabetes Research and Clinical Practice·J SatoN Sakamoto
Mar 21, 2006·Experimental Parasitology·Claudia MachucaAlicia Ponte-Sucre

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