In Vitro Effects of the Endocrine Disruptor p,p'-DDT on Human Follitropin Receptor

Environmental Health Perspectives
Mathilde MunierPatrice Rodien

Abstract

1-chloro-4-[2,2,2-trichloro-1-(4-chlorophenyl)ethyl]benzene (p,p'-DDT) is a persistent environmental endocrine disruptor (ED). Several studies have shown an association between p,p'-DDT exposure and reproductive abnormalities. To investigate the putative effects of p,p'-DDT on the human follitropin receptor (FSHR) function. We used Chinese hamster ovary (CHO) cells stably expressing human FSHR to investigate the impact of p,p'-DDT on FSHR activity and its interaction with the receptor. At a concentration of 5 μM p,p'-DDT increased the maximum response of the FSHR to follitropin by 32 ± 7.45%. However, 5 μM p,p'-DDT decreased the basal activity and did not influence the maximal response of the closely related LH/hCG receptor to human chorionic gonadotropin (hCG). The potentiating effect of p,p'-DDT was specific for the FSHR. Moreover, in cells that did not express FSHR, p,p'-DDT had no effect on cAMP response. Thus, the potentiating effect of p,p'-DDT was dependent on the FSHR. In addition, p,p'-DDT increased the sensitivity of FSHR to hCG and to a low molecular weight agonist of the FSHR, 3-((5methyl)-2-(4-benzyloxy-phenyl)-5-{[2-[3-ethoxy-4-methoxy-phenyl)-ethylcarbamoyl]-methyl}-4-oxo-thiazolidin-3-yl)-benzamide (16a). Basal ...Continue Reading

References

Mar 29, 1991·Biochemical and Biophysical Research Communications·T MinegishiT ] Minegish T [corrected to Minegishi
May 1, 1994·The Journal of Endocrinology·T MinegishiY Ibuki
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Feb 24, 2001·European Journal of Pediatric Surgery : Official Journal of Austrian Association of Pediatric Surgery ... [et Al] = Zeitschrift Für Kinderchirurgie·S HosieK L Waag
Jul 5, 2003·Lancet·Barbara A CohnPentti K Siiteri
Aug 9, 2003·Molecular Endocrinology·Hanumanthappa KrishnamurthyMario Ascoli
Aug 22, 2003·The New England Journal of Medicine·Guillaume SmitsSabine Costagliola
Mar 6, 2004·The Journal of Clinical Endocrinology and Metabolism·Lucia MontanelliSabine Costagliola
Mar 9, 2004·Trends in Biochemical Sciences·Gilbert VassartSabine Costagliola
May 8, 2004·Human Reproduction·O VasiliuW Karmaus
Feb 11, 2005·Epidemiology·Gayle C WindhamBill Lasley
Aug 27, 2005·American Journal of Epidemiology·Scott A VennersXiaobin Wang
Nov 22, 2005·Occupational and Environmental Medicine·F OuyangX Wang
Jan 20, 2006·Journal of Medicinal Chemistry·Woody ShermanRamy Farid
Feb 24, 2006·Chemical Biology & Drug Design·Woody ShermanRamy Farid
Mar 17, 2006·The Journal of Biological Chemistry·Stephen D YanofskyRonald W Barrett
May 6, 2006·Bioorganic & Medicinal Chemistry·Jay WrobelEmily S Shen
Jul 13, 2006·Environmental Health Perspectives·Ida N DamgaardUNKNOWN Nordic Cryptorchidism Study Group
Oct 26, 2006·The Journal of Pharmacology and Experimental Therapeutics·Mario RossiRoberto Maggio
Feb 5, 2008·Endocrine·Alfredo Ulloa-AguirreJames A Dias
Feb 7, 2008·Biochemical and Biophysical Research Communications·Brian J AreyRichard C Winneker
Feb 19, 2009·Clinical Endocrinology·Christine Wohlfahrt-VejeNiels Erik Skakkebaek
May 4, 2010·Asian Journal of Andrology·Hao WangXiao-Meng Li
Sep 28, 2010·Trends in Pharmacological Sciences·Mette M RosenkildeThue W Schwartz
Dec 28, 2010·Molecular and Cellular Endocrinology·James A DiasBrice Campo
Aug 27, 2011·Methods in Molecular Biology·Brock F BinkowskiKeith V Wood
Sep 9, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Thaddeus T SchugJerrold J Heindel
Jan 1, 2011·Frontiers in Endocrinology·Pauline GloaguenEric Reiter
Jul 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Xuliang JiangXiaolin He
Aug 22, 2012·Biodegradation·Simi SudharshanNanthi Bolan

❮ Previous
Next ❯

Citations

Feb 13, 2020·Current Medical Research and Opinion·Ilaria SoaveDonatella Caserta
Nov 14, 2019·Nature Reviews. Endocrinology·Michele A La MerrillR Thomas Zoeller
Feb 23, 2020·Frontiers in Pharmacology·Messaouda Merzoug-LarabiJean-Marc Ricort
Feb 5, 2021·Journal of Environmental Management·Elizabeth R RogersChung-Ho Lin
Aug 26, 2020·Molecular and Cellular Endocrinology·Jones B GraceliAnna C Denicol

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
FCS

Software Mentioned

Prism
Propka
Epik
Schrödinger Suite
Impact
GraphPad
MODELLER
Prime
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.