In Vitro Effects of Thymol-β-D-Glucopyranoside on Salmonella enterica Serovar Typhimurium and Escherichia coli K88

Journal of Food Protection
G LeventDavid J Nisbet

Abstract

Although thymol is bactericidal against many pathogens in vitro, its in vivo effectiveness against pathogens in the lower gastrointestinal tract is limited because of its rapid absorption in the proximal gut. Thymol-β-<small>D</small>-glucopyranoside (β-thymol), a conjugated form of thymol, can deliver thymol to the lower gastrointestinal tract and has shown antibacterial effects. In the present study, we examined the in vitro effects of β-thymol on Salmonella enterica serovar Typhimurium (ST) and Escherichia coli K88 (K88). We inoculated one-half strength Mueller-Hinton broth with 5.8 ± 0.09 log CFU/ml novobiocin- and naladixic acid-resistant (NN) ST (NVSL 95-1776) and 5.1 ± 0.09 log CFU ml(-1) NN-resistant K88, with or without porcine feces (0.1% [wt/vol]) (fecal incubations). The resultant bacterial suspensions were distributed under N2 to triplicate sets of tubes to achieve initial concentrations of 0, 3, 6, and 12 mM for ST treatments and 0, 3, 12, and 30 mM for K88 treatments. Samples were incubated at 39°C and then plated onto NN-containing brilliant green agar and NN-containing MacConkey agar; ST and K88 CFU concentrations were determined via 10-fold dilutions, and viable cell counts were performed at 0, 6, and 24 h. No...Continue Reading

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Citations

Feb 26, 2020·Animals : an Open Access Journal From MDPI·Noémie Van NotenJoris Michiels
Feb 18, 2020·Journal of Animal Science·Claudio Arzola-AlvarezJaime Salinas-Chavira
Apr 26, 2020·Journal of Animal Physiology and Animal Nutrition·Noémie Van NotenJoris Michiels
Mar 24, 2020·ACS Omega·Noémie Van NotenJoris Michiels

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