In vitro effects of two major phenolic compounds from the family Lamiaceae plants on the human gastric carcinoma cells

Toxicology and Industrial Health
Ayse Günes-BayirEray Metin Guler

Abstract

Phenolic compounds of essential oils from the family Lamiaceae are commonly used substances in the food industry because of their flavouring, antimicrobial and antioxidant properties. In this context, it has become important to have healthy and safe products for consumers who are exposed to these phenolic compounds. The present study was aimed to investigate the toxic effects of carvacrol, thymol and their mixture on human gastric carcinoma (AGS) cells. Cells were analyzed after 24 h of exposure to different concentrations of carvacrol, thymol and their mixture by the ATP cell viability, 2',7' dichlorodihydrofluorescein diacetate (H2DCF-DA), reducte glutatione/oxide glutathione ((GSH)/GSSG-Glo) and comet assays. Apoptosis induction was studied by acridine orange/ethidium bromide staining and western blotting. Carvacrol, thymol and their mixture induced cytotoxicity, genotoxicity, apoptosis, increased reactive oxygen species (ROS) and decreased GSH levels after 24 h of their exposure in a dose-dependent manner. A close negative relationship was found between cell viability and ROS generation. We examined dose-dependent cytotoxic effects of carvacrol, thymol and their mixture in human AGS cells. Increased intracellular ROS causes...Continue Reading

References

Feb 1, 1975·Journal of the American Oil Chemists' Society·A L Branen
Aug 1, 1987·Pharmacology & Toxicology·L T AustgulenR R Scheline
Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Apr 1, 1967·Food and Cosmetics Toxicology·E C HaganJ B Brouwer
Sep 18, 1999·Free Radical Biology & Medicine·H Wang, J A Joseph
Oct 3, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A StammatiA von Wright
Nov 7, 2001·Current Treatment Options in Cardiovascular Medicine·Peter J. StokmanRichard W. Asinger
Dec 11, 2002·Mutagenesis·A HartmannUNKNOWN 4th International Comet Assay Workshop
Apr 16, 2004·Journal of Food Protection·Arzu CagriElliot T Ryser
May 25, 2004·Infection and Immunity·Stefan MoeseSteffen Backert
Dec 24, 2005·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·K VermeireP H Van de Heyning
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·S Li, E H Blackburn
Nov 13, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F BakkaliM Idaomar
Apr 25, 2008·Current Protocols in Immunology·W Strober
May 30, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·U UndeğerN Başaran
Jan 26, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·K M Arunasree
Mar 18, 2011·International Journal of Food Microbiology·Abel GuardaMaria Jose Galotto
Jun 7, 2011·Chemico-biological Interactions·Dipanwita Dutta DebTapan Chakraborty
Sep 15, 2011·European Journal of Pharmacology·Shu-Shong HsuChung-Ren Jan
Jan 1, 2006·CSH Protocols·Shailaja KasibhatlaDouglas R Green
Jan 1, 2006·CSH Protocols·Shailaja KasibhatlaDouglas R Green
Jan 1, 2006·CSH Protocols·Shailaja KasibhatlaDouglas R Green
Dec 12, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·María Llana-Ruiz-CabelloAna María Cameán
Jul 20, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Maria LLana-Ruiz-CabelloAna M Cameán
Feb 25, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·María Llana-Ruiz-CabelloAna María Cameán
Apr 14, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Maria Llana-Ruiz-CabelloAna M Cameán
Oct 7, 2015·Journal of Microbiology and Biotechnology·Seo-Hee KangPyo-Jam Park
Jan 10, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M Llana-Ruiz-CabelloA M Cameán

❮ Previous
Next ❯

Citations

Apr 25, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sabrina AdorisioMaria Carla Marcotullio
Jul 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ayse Günes-BayirAgnes Dadak
Jul 27, 2021·Frontiers in Pharmacology·Laeza Alves SampaioAdriana Gibara Guimarães
Jul 26, 2018·Journal of Agricultural and Food Chemistry·Liang-Bin HuXiaohui Zhou

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
electrophoresis

Software Mentioned

Statistical Package for the Social Sciences

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2022 Meta ULC. All rights reserved