In vitro efficacy of diallyl sulfides against the periodontopathogen Aggregatibacter actinomycetemcomitans.

Antimicrobial Agents and Chemotherapy
K VelliyagounderD H Fine

Abstract

The in vitro antibacterial effects of diallyl sulfide (DAS) against the Gram-negative periodontopathogen Aggregatibacter actinomycetemcomitans, the key etiologic agent of the severe form of localized aggressive periodontitis and other nonoral infections, were studied. A. actinomycetemcomitans was treated with garlic extract, allicin, or DAS, and the anti-A. actinomycetemcomitans effects of the treatment were evaluated. Garlic extract, allicin, and DAS significantly inhibited the growth of A. actinomycetemcomitans (greater than 3 log; P < 0.01) compared to control cells. Heat inactivation of the garlic extracts significantly reduced the protein concentration; however, the antimicrobial effect was retained. Purified proteins from garlic extract did not exhibit antimicrobial activity. Allicin lost all its antimicrobial effect when it was subjected to heat treatment, whereas DAS demonstrated an antimicrobial effect similar to that of the garlic extract, suggesting that the antimicrobial activity of garlic extract is mainly due to DAS. An A. actinomycetemcomitans biofilm-killing assay performed with DAS showed a significant reduction in biofilm cell numbers, as evidenced by both confocal microscopy and culture. Scanning electron mic...Continue Reading

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Citations

Oct 17, 2014·Journal of Applied Microbiology·K VelliyagounderD H Fine
Dec 3, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Xy LanZy Tang
Nov 30, 2019·Toxicology Mechanisms and Methods·Shereen M GalalAbeer A Elkhoely
Feb 6, 2020·Experimental and Therapeutic Medicine·Masahiro Ohtani, Tsubasa Nishimura
Aug 17, 2021·Frontiers in Microbiology·Sushma Bagde BhatwalkarRajaneesh Anupam

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