PMID: 19912757Aug 1, 1990Paper

In Vitro electrophysiology of dorsal raphe serotonergic neurons in subchronic cocaine-treated rats: Development of tolerance to acute cocaine administration

Molecular and Cellular Neurosciences
E W Black, J M Lakoski

Abstract

An in vitro, extracellular slice preparation was used to examine the effects of subchronic cocaine administration on serotonergic cell firing in the dorsal raphe. Male Sprague-Dawley rats were administered cocaine (30 mg/kg, ip) for 7 days prior to experimentation. Following a 24-h washout period, rats were decapitated and dorsal raphe nucleus slices (400 mum) obtained. Slices were superfused with ACSF containing 1 AM phenylephrine and 1 muM 5-hydroxytryptamine (5-HT). Acute cocaine application was found to inhibit basal firing rates in both saline- and cocaine-pretreated rats. In addition, an apparent tolerance to acute cocaine application was found in animals subchronically treated with cocaine. Animals exposed to repeated cocaine exposure required an acute cocaine concentration of 5.46 +/- 0.5 muM (N = 5) to inhibit cell basal firing rates by 50% versus concentrations of 2.43 +/- 0.35 muM (N = 5) for saline controls. Concentrations of 20 muM cocaine typically produced complete inhibition of firing in cocaine-pretreated animals, whereas 10 muM cocaine produced complete cessation of firing in saline controls. Repeated perfusion with equal concentrations of cocaine produced dose-dependent inhibition of firing which remained con...Continue Reading

Citations

Jul 1, 1992·Pharmacology, Biochemistry, and Behavior·A D LevyL D Van de Kar
Mar 1, 1992·Pharmacology, Biochemistry, and Behavior·N A DarmaniR A Glennon

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