In vitro embryotoxicity testing of metals for dental use by differentiation of embryonic stem cell test

Congenital Anomalies
Koichi Imai, Masaaki Nakamura

Abstract

We examined embryotoxicity using the embryonic stem cell test (EST) protocol. Tests were conducted using standard reagents for the atomic absorption measurement of 11 metal ions, silver, cobalt, chromium, copper, mercury, nickel, palladium, antimony, tin, vanadium, and zinc from among metals comprising dental alloys. In addition, for four metals like silver, cobalt, chromium, and nickel, the tests were also conducted using a test solution extracted from powder in the cell culture medium. The embryotoxic potential was obtained from a biostatistics-based prediction model, which was calculated from three endpoints, the ID50, IC50ES and IC(50)3T3. Data with the standard reagents showed that chromium and mercury ions corresponded to class 3, that is, having a strong embryotoxicity, while antimony, tin, and vanadium ions exhibited a weak embryotoxicity. The other metal ions demonstrated no embryotoxicity. On the other hand, when extracts of metal powder in cell culture solutions were used, silver exhibited a weak embryotoxicity while all other metals exhibited no embryotoxicity. In the future, it will be important to clarify the embryotoxicity of the many dental materials that are in use today. In addition, it is necessary to develop...Continue Reading

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Citations

Jan 14, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Christian RiebelingAndrea Seiler
Jan 27, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Roland BuesenAndrea Seiler
Oct 4, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Noriyuki SuzukiKoichi Saito
Jan 27, 2010·Toxicology Mechanisms and Methods·Rui ChenAndy Peng Xiang
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Lorena Fernandez-GonzalezKatarina Jewgenow

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